| Project/Area Number |
26891020
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Miyagi University |
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Principal Investigator |
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 先端成長 / 微小管 / アクチン繊維 / コケ植物 / フォルミン / ライブイメージング / アクチン重合促進因子 / 微小管付随タンパク質 |
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| Outline of Final Research Achievements |
Tip-growing cells display polarized cell growth. Microtubules (MTs) and actin filaments (AFs) have been implicated in regulating directionality and expansion. However, the regulatory interaction between MTs and AFs in tip-growing plant cells remains unclear. In this project, we showed that the proper generation of AFs was likely dependent on the existing MTs in the polarized expansion zone of caulonemal cells of the moss Physcomitrella patens. Moreover, the actin polymerizing factor formins, For2A and 2B, were involved in the regulation of the rate of cell growth. The live-imaging technique revealed that AF foci were generated in the existing MT foci in the expansion zone. The MT depolymerization led to the loss of the AF foci, suggesting that the AF foci are formed on the MT foci. The inducible RNAi screening of the putative MT-AF regulators indicated that For2A and 2B played roles on promoting the expansion rate.
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