Molecular mechanism of melanopsin to induce non-image forming responses

• Project/Area Number: 26891022
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Animal physiology/Animal behavior
• Research Institution: Keio University
• Principal Investigator: Hatori Megumi 慶應義塾大学, 医学部, 准教授 (90590472)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: メラノプシン / 概日リズム / 網膜 / 光受容

Outline of Final Research Achievements

Melanopsin photopigment expressed in intrinsically photosensitive retinal ganglion cells plays a crucial role in the adaptation of mammals to ambient light environment through both image-forming and non-image-forming visual responses. The melanopsin-expressing retinal ganglion cells are structurally and functionally distinct from classical rod/cone photoreceptors and have unique properties, including single-photon response, long response latency, photon integration over time, and slow deactivation. To understand the molecular property of melanospin, I generated various mutant versions of melanopsin, and these proteins were expressed in mammalian cells. Blue light-dependent increases in cytosolic calcium were monitored. By using this assay system, several of mutant melanopsin were obtained to show in vitro response difference compared to wild type melanopsin, and the effect of these mutant melanopsin on non-image forming responses will be evaluated in vivo in mice.

Research Products

• [Journal Article] 時計システムと老化 (2016)
  • Author(s): 津山 淳、羽鳥 恵
  • Journal Title: 日本臨床 Volume: -
• [Journal Article] ブルーライト研究の歴史と概日時計との関わり (2016)
  • Author(s): 羽鳥 恵
  • Journal Title: ブルーライトテキストブック Volume: -
• [Journal Article] 網膜 - 視床下部の光応答 (2016)
  • Author(s): 羽鳥 恵
  • Journal Title: 光と生命の事典 Volume: - Pages: 214-215
• [Journal Article] Response of peripheral rhythms to the timing of food intake. (2015)
  • Author(s): Hatori M and Panda S
  • Journal Title: Methods in Enzymology Volume: 552 Pages: 145-161
  • DOI: 10.1016/bs.mie.2014.10.027
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 体内時計への入力 (2015)
  • Author(s): 羽鳥 恵
  • Journal Title: Medical Science Digest Volume: 41 Pages: 382-385
• [Journal Article] 体内時計と眼 (2015)
  • Author(s): 羽鳥 恵、坪田 一男
  • Journal Title: アンチ・エイジング医学 Volume: 11 Pages: 385-392
  • NAID: 40020527265
• [Journal Article] 概日リズムと老化 (2015)
  • Author(s): 羽鳥 恵
  • Journal Title: 第三版アンチエイジング医学の基礎と臨床 Volume: - Pages: 65-66
• [Journal Article] 眼と時間生物学 (2015)
  • Author(s): 羽鳥 恵
  • Journal Title: 眼科研修ノート 改訂第2版 Volume: - Pages: 445-447
• [Presentation] 概日リズムと老化 (2016)
  • Author(s): 羽鳥 恵
  • Organizer: 日本抗加齢医学会
  • Place of Presentation: 大手町サンケイプラザ（東京都千代田区）
  • Year and Date: 2016-03-27
  • Invited
• [Presentation] 概日時計と外界環境との調和機構 (2015)
  • Author(s): 羽鳥 恵
  • Organizer: 第8回Symphony
  • Place of Presentation: ホテルメトロポリタン エドモント（東京都千代田区）
  • Year and Date: 2015-09-27
  • Invited
• [Presentation] 体内時計の理解と生活習慣病やブルーライト弊害の予防 (2015)
  • Author(s): 羽鳥 恵
  • Organizer: 第８回日本薬学会関東支部若手シンポジウム
  • Place of Presentation: 日本大学薬学部キャンパス（千葉県船橋市）
  • Year and Date: 2015-09-12
  • Invited
• [Presentation] 時間生物学の基礎―メラノプシンと概日リズム (2015)
  • Author(s): 羽鳥 恵
  • Organizer: Cataract Academic Seminar
  • Place of Presentation: ＪＰタワー ホール＆カンファレンス（東京都千代田区）
  • Year and Date: 2015-08-02
  • Invited
• [Presentation] The Circadian Clock and Melanopsin: a blue-light sensitive photopigment in mammalian retinal ganglion cells (2015)
  • Author(s): Megumi Hatori
  • Organizer: Asia-ARVO 2015
  • Place of Presentation: 横浜
  • Year and Date: 2015-02-16
• [Presentation] 白内障と睡眠障害 (2014)
  • Author(s): 羽鳥 恵
  • Organizer: 一般財団法人白内障研究所 白内障研究所市民公開講座
  • Place of Presentation: 東京
  • Year and Date: 2014-10-11