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Analysis of the mechanisms by which the extracellular dsRNA systemically spreads.

Research Project

Project/Area Number 26891023
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionTokyo Women's Medical University

Principal Investigator

Dejima Katsufumi  東京女子医科大学, 医学部, 助教 (60457439)

Project Period (FY) 2014-08-29 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords全身性RNAi / 細胞外RNA
Outline of Final Research Achievements

In C. elegans, dsRNA can systemically spread throughout the animal and lead to non-cell autonomous RNAi in cells distinct from the cells where dsRNA is originally introduced. This RNA silencing phenomenon is called the “systemic RNAi”. Systemic RNAi is thought to largely depend on vesicle transport because clathrin-dependent endocytosis and several proteins that localize endomembrane systems have been shown to be involved in systemic RNAi. However, underlying molecular mechanisms by which dsRNA is secreted to the extracellular space and enters into cells still unclear. To identify new factors that is involved in systemic dsRNA spread, genetic screening in which a strain defective in systemic RNAi restores RNAi ability was performed using C. elegans. One of the causal genes of the mutants was identified and analyzed.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • Research Products

    (3 results)

All 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results,  Open Access: 1 results) Presentation (1 results)

  • [Journal Article] Syndecan defines precise spindle orientation by modulating Wnt signaling in C. elegans.2014

    • Author(s)
      Dejima K, Kang S, Mitani S, Cosman PC, Chisholm AD.
    • Journal Title

      Development

      Volume: 141 Issue: 22 Pages: 4354-65

    • DOI

      10.1242/dev.113266

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C. elegans glycogene database.2014

    • Author(s)
      Akiyoshi S, Nomura KH, Dejima K, Murata D, Matsuda A, Kanaki N, Takaki T, Mihara H, Nagaishi T, Furukawa S, Ando KG, Yoshina S, Mitani S, Togayachi A, Suzuki Y, Shikanai T, Narimatsu H, Nomura K.
    • Journal Title

      Glycobiology

      Volume: 25(1) Issue: 1 Pages: 8-20

    • DOI

      10.1093/glycob/cwu080

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 線虫C. elegans における全身性RNAiを負に制御する因子の探索2014

    • Author(s)
      出嶋 克史、今江 理恵子、三谷 昌平
    • Organizer
      第37回日本分子生物学会年会会期
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report

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Published: 2014-09-09   Modified: 2017-05-10  

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