Evaluation of correlation between rotation and secretion of type III secretion apparatus for clarifying the effector secretion mechanism
| Project/Area Number |
26891024
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Kyoto Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 細菌Ⅲ型分泌装置 / 緑膿菌 / 回転-分泌相関 / エフェクター輸送速度測定 / 回転運動観察 / エフェクター分泌 / 蛍光標識エフェクター / 金ナノロッド |
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| Outline of Final Research Achievements |
Bacteria like Pseudomonas aeruginosa inject effector proteins, which contribute to bacterial infection, into host cytosol via needle-like type III secretion machinery (T3SA). However, the transport mechanism of effectors in T3SA is unclear. In this study, we measured the speed of effector transport in T3SA. The measured speed of effector transport in T3SA showed correlation with the speed of T3SA rotation previously observed. Consequently, it was suggested that effecters are transported in T3SA needle by the rotation of T3SA.
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Report
(3 results)
Research Products
(12 results)
