| Project/Area Number |
26891028
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Ito Yoko 国立研究開発法人理化学研究所, 光量子工学研究領域, 特別研究員 (10733016)
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | ゴルジ体 / 膜交通 / ライブイメージング / 植物 |
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| Outline of Final Research Achievements |
The Golgi apparatus, one of the single-membrane-bounded organelles, plays essential roles in eukaryotic cells. By inhibiting ER-Golgi transport by a chemical compound BFA, the Golgi apparatus disappears and specific proteins which localize to the cis-most cisternae accumulate in unknown punctate structures. We have found that this localization of the cis-Golgi proteins is achieved by the transport route which is not inhibited by BFA and independent of COPII machinery. We have also demonstrated by 3D time-lapse observations that the punctate structures receive cargoes from the ER during Golgi regeneration after BFA removal. In addition, our observation revealed that the number of the Golgi stacks per cell, which was long believed to be regulated by the cell cycle, is strongly related to the cell growth, not to the cell cycle itself.
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