Construction of experimental method for SRK-SCR interaction by using an SRK protein from plant cells.
| Project/Area Number |
26892004
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
Yamamoto Masaya 東北大学, (連合)農学研究科(研究院), 助教 (70732543)
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 自家不和合性 / アブラナ科植物 / 分子細胞生物学 / ペプチド-リガンド相互作用 / 受容体-リガンド相互作用 |
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| Outline of Final Research Achievements |
The Brassicaceae plants possess the self-incompatible mechanism to avoid a self-fertilization. SRK, which is a receptor kinase in the stigma cell, and SCR, which is a pollen coat-localized ligand, play a major role in self-incompatible response. Although SRK-SCR interaction is important for self-incompatibility, its molecular mechanism remains unclear. Therefore, I made to construct the experimental method for analysis of SRK-SCR interaction. For this purpose, I developed preparation methods for SRK protein from A. thaliana cells and SCR protein from E. coli cells. By using SCR protein, we analyzed a BoSCR-45, and found an interested property. I would like to investigate the molecular mechanism of SRK-SCR interaction by using developed method in this work.
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Report
(3 results)
Research Products
(9 results)
