| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
This study carried out basic research for vaccine development by using non-genetically-modified lactic acid bacteria (LAB). A recombinant protein containing GFP (reporter part) and LysM (anchor part) was prepared by E. coli expression system and was displayed on the cell surface of LAB by co-incubation. The amount of displayed protein could be simply and quickly quantified by measuring the fluorescence intensity emitted by the LAB cells treated. This simple quantification method made it possible to assess various experimental conditions such as the preparation of LAB cells, conditions for displaying proteins, and the sustainability of displayed proteins. It was also demonstrated that this cell surface display system is useful not only for L. lactis but also for many other species of LAB.
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