Development of H. pylori vaccine based on lactic acid bacteria

• Project/Area Number: 26892011
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: IWATANI SHUN 東京工業大学, 生命理工学研究科, 助教 (80608373)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 乳酸菌ワクチン / 細胞表層ディスプレイ / ヘリコバクター・ピロリ / 乳酸菌 / ワクチン / 抗原提示 / 感染防御

Outline of Final Research Achievements

This study carried out basic research for vaccine development by using non-genetically-modified lactic acid bacteria (LAB). A recombinant protein containing GFP (reporter part) and LysM (anchor part) was prepared by E. coli expression system and was displayed on the cell surface of LAB by co-incubation. The amount of displayed protein could be simply and quickly quantified by measuring the fluorescence intensity emitted by the LAB cells treated. This simple quantification method made it possible to assess various experimental conditions such as the preparation of LAB cells, conditions for displaying proteins, and the sustainability of displayed proteins. It was also demonstrated that this cell surface display system is useful not only for L. lactis but also for many other species of LAB.

Research Products

• [Journal Article] Identification and functional characterization of Penicillium marneffei pleiotropic drug resistance transporters ABC1 and ABC2. (2016)
  • Author(s): Panapruksachat S, Iwatani S, Oura T, Vanittanakom N, Chindamporn A, Niimi K, Niimi M, Lamping E, Cannon RD, Kajiwara S.
  • Journal Title: Medical Mycology Volume: in press
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Toll-like Receptor 10 in Helicobacter pylori Infection (2015)
  • Author(s): Nagashima H, Iwatani S, Cruz M, Jiménez Abreu JA, Uchida T, Mahachai V, Vilaichone RK, Graham DY, Yamaoka Y
  • Journal Title: J Infect Dis Volume: 212 Issue: 10 Pages: 1666-1676
  • DOI: 10.1093/infdis/jiv270
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Differences in interleukin 8 expression in Helicobacter pylori-infected gastric mucosa tissues from patients in Bhutan and the Dominican Republic (2015)
  • Author(s): Nagashima H, Iwatani S, Cruz M, Jiménez Abreu JA, Tronilo L, Rodríguez E, Disla M, Terao H, Uchida T, Mahachai V, Vilaichone RK, Tshering L, Mitsui T, Shiota S, Graham DY, Yamaoka Y
  • Journal Title: Hum Pathol Volume: 46 Issue: 1 Pages: 129-136
  • DOI: 10.1016/j.humpath.2014.10.006
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Gene cluster responsible for secretion of and immunity to multiple bacteriocins, the NKR-5-3 enterocins (2014)
  • Author(s): Ishibashi N, Himeno K, Masuda Y, Perez RH, Iwatani S, Zendo T, Wilaipun P, Leelawatcharamas V, Nakayama J, Sonomoto K
  • Journal Title: Appl Environ Microbiol Volume: 80 Issue: 21 Pages: 6647-6655
  • DOI: 10.1128/aem.02312-14
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification of the genes that contribute to lactate utilization in Helicobacter pylori. (2014)
  • Author(s): Iwatani S, Nagashima H, Reddy R, Shiota S, Graham DY, Yamaoka Y
  • Journal Title: PLoS One Volume: 9 Issue: 7 Pages: 103506-103506
  • DOI: 10.1371/journal.pone.0103506
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Virulence genes of Helicobacter pylori in the Dominican Republic (2014)
  • Author(s): Shiota S, Cruz M, Abreu JA, Mitsui T, Terao H, Disla M, Iwatani S, Nagashima H, Matsuda M, Uchida T, Tronilo L, Rodríguez E, Yamaoka Y
  • Journal Title: J Med Microbiol Volume: 63 Issue: 9 Pages: 1189-1196
  • DOI: 10.1099/jmm.0.075275-0
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Discovery of novel mutations for clarithromycin resistance in Helicobacter pylori by using next-generation sequencing (2014)
  • Author(s): Binh TT, Shiota S, Suzuki R, Matsuda M, Trang TT, Kwon DH, Iwatani S, Yamaoka Y
  • Journal Title: J Antimicrob Chemother Volume: 69 Issue: 7 Pages: 1796-1803
  • DOI: 10.1093/jac/dku050
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Candida ssp. induces the apoptosis of human hepatic cells by reactive oxygen species (ROS) (2016)
  • Author(s): Susumu Kajiwara, Ronak Shrestha, Rajan Shrestha, Xian-Yang Qin, Yugo Oshima, Shun Iwatani, Adusa Nakagushi-Takahashi, Hiroji Chibana, Reizo Kato, Soichi Kojima.
  • Organizer: 第89回日本細菌学会
  • Place of Presentation: 大阪国際交流センター
  • Year and Date: 2016-03-23
• [Presentation] Biosynthesis and antimicrobial mechanisms of a leaderless bacteriocin, lacticin Q (2015)
  • Author(s): Takeshi Zendo, Shiho Ogata, Mengqi Li, Naoki Ishibashi, Yoshimitsu Masuda, Shun Iwatani, Fuminori Yoneyama, Jiro Nakayama, Kenji Sonomoto
  • Organizer: The 8th Asian Conference on LACTIC ACID BACTERIA
  • Place of Presentation: the Emerald Hotel, Bangkok, Thailand
  • Year and Date: 2015-07-08
  • Int'l Joint Research
• [Presentation] Toll-Like Receptor 10 in Helicobacter pylori Infection (2015)
  • Author(s): Hiroyuki Nagashima, Shun Iwatani, Shingo Tanaka, Takahiro Uotani, Modesto Cruz, Jose A. Jimenez Abreu, Tomohisa Uchida, Varocha Mahachai, Ratha-korn Vilaichone, David Y. Graham, Yoshio Yamaoka
  • Organizer: Digestive Disease Week 2015
  • Place of Presentation: Walter E. Washington Convention Center, Washington DC, USA
  • Year and Date: 2015-05-17
  • Int'l Joint Research
• [Presentation] リーダーレスバクテリオシン、ラクティシンQの生合成および抗菌機構 (2014)
  • Author(s): 善藤 威史、緒方 詩保、Mengqi Li、石橋 直樹、益田 時光、岩谷 駿、米山 史紀、園元 謙二
  • Organizer: 日本生物工学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-09 – 2014-09-11