| Project/Area Number |
26892024
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Toyama Prefectural University |
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Principal Investigator |
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 細胞外DNA / プラスミド / 富山湾 |
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| Outline of Final Research Achievements |
Using two filters to remove cells and viral particles followed by several steps of concentration and purification, we succeeded in obtaining metagenome sequences of extracellular DNA from three surface seawater and four marine sediment sampled at different sites on Toyama Bay, Japan. G+C content and oligonucleotide frequency profiles showed that the composition of extracellular DNAs from seawater were similar to the viral DNA isolated from the same sample, while those from sediments were similar to the intracellular DNA. The ratio of reads showing similarity (blastn, e-value <1e-5) with already-known plasmid (9225 sequences in RefSeq v74) were ranged from 0.2-1.0%, and had some correlation with those having similarity with bacterial sequence (3-16%). Some plasmid-derived sequences were only detected in extracellular DNA, indicating that the composition of extracellular plasmid is not the same with that of intracellular plasmid.
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