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An endogenous protein inhibitor, YjhX (TopI), for topoisomerase I from Escherichia coli

Research Project

Project/Area Number 26892025
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionOsaka City University

Principal Investigator

Yamaguchi Yoshihiro  大阪市立大学, 複合先端研究機構, 特任准教授(テニュアトラック) (00737009)

Project Period (FY) 2014-08-29 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords応用微生物 / バイオテクノロジー / 生化学 / 分子生物学 / 分子生物 / 応用微生物学 / 酵素学 / 遺伝子工学
Outline of Final Research Achievements

Here, I found a novel, previously unidentified TA system in Escherichia coli named yjhX-yjhQ. Induction of YjhX (85 amino acid residues) causes cell-growth arrest resulting in cell death, while YjhQ (181 residues) co-induction resumes cell growth. The primary cellular target of YjhX was found to be topoisomerase I (TopA), inhibiting both DNA replication and RNA synthesis. Notably, YjhX has no homology to any other toxins of the TA systems. YjhX was expressed well with an N-terminal protein S (PrS) tag in soluble forms. PrS-YjhX specifically interacts with the N-terminal region of TopA (TopA67) but not full-TopA in the absence of plasmid DNA, while PrS-YjhX binds to full-TopA in the presence of DNA. Notably, YjhX does not directly interact with DNA and RNA. YjhX inhibits only topoisomerase I but not topoisomerase III and IV in vitro. Hence, yjhX is renamed as the gene for the topoisomerase I inhibitor (the topI gene). TopI is the first protein inhibitor specific for topoisomerase I.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • Research Products

    (2 results)

All 2015

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (1 results)

  • [Journal Article] An endogenous protein inhibitor, YjhX (TopAI), for topoisomerase I from Escherichia coli.2015

    • Author(s)
      Yoshihiro Yamaguchi and Masayori Inouye
    • Journal Title

      Nucleic Acids Research

      Volume: 43 Pages: 10387-10396

    • DOI

      10.1093/nar/gkv1197

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] SymE, a hexameric E. coli toxin, is a DNA gyrase inhibitor in E. coli2015

    • Author(s)
      山口良弘
    • Organizer
      日本農芸化学会
    • Place of Presentation
      岡山大学( 岡山県岡山市 )
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Annual Research Report

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Published: 2014-09-09   Modified: 2017-05-10  

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