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Mechanism of Ebola viral transcription and replication

Research Project

Project/Area Number 26892028
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Veterinary medical science
Research InstitutionOkinawa Institute of Science and Technology Graduate University

Principal Investigator

Yukihiko Sugita  沖縄科学技術大学院大学, その他の研究科, 研究員 (00734469)

Project Period (FY) 2014-08-29 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsエボラウイルス / クライオ電子顕微鏡 / ウイルス学 / 電子顕微鏡
Outline of Final Research Achievements

In this study, we aimed to further understanding of structural basis of Ebola viral transcription and replication by an cryo-electron microscopic analysis of the viral nucleocapsid core, nucleoprotein-RNA complex (NP helix). The reconstructed three-dimensional structure of the NP helix shows that it is formed by a helical assembly of a band of tightly packed NP units. The clearly resolved alpha-helical structures of NP C-terminus are located at the seam between the axial boundaries of the protein band. The structure suggests both a mechanism of self-assembly and reversible structural change of the NP helix during the Ebola viral life cycle.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • Research Products

    (7 results)

All 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Genetic and antigenic characterization of bovine viral diarrhea viruses isolated from cattle in Hokkaido, Japan2016

    • Author(s)
      Abe Y, Tamura T, Torii S, Wakamori S, Nagai M, Mitsuhashi K, Mine J, Fujimoto Y, Nagashima N, Yoshino F, Sugita Y, Nomura T, Okamatsu M, Kida H, Sakoda Y
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 78 Issue: 1 Pages: 61-70

    • DOI

      10.1292/jvms.15-0186

    • NAID

      130005121585

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Actin-Modulating Protein Cofilin Is Involved in the Formation of Measles Virus Ribonucleoprotein Complex at the Perinuclear Region2015

    • Author(s)
      Koga R, Sugita Y, Noda T, Yanagi Y, Ohno S.
    • Journal Title

      Journal of Virology

      Volume: 89 Issue: 20 Pages: 10524-10531

    • DOI

      10.1128/jvi.01819-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Influenza virus-host interactome screen as a platform for antiviral drug development.2014

    • Author(s)
      Watanabe T, Kawakami E, Shoemaker JE, Lopes TJ, Matsuoka Y, Tomita Y, Kozuka-Hata H, Gorai T, Kuwahara T, Takeda E, Nagata A, Takano R, Kiso M, Yamashita M, Sakai-Tagawa Y, Katsura H, Nonaka N, Fujii H, Fujii K, Sugita Y, Noda T, Goto H, Fukuyama S, Watanabe S, Neumann G, Oyama M, Kitano H, Kawaoka Y.
    • Journal Title

      Cell Host and Microbe

      Volume: 16 Issue: 6 Pages: 795-805

    • DOI

      10.1016/j.chom.2014.11.002

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Cryo-electron tomographic analysis of influenza viral transcription2016

    • Author(s)
      Yukihiko Sugita
    • Organizer
      Three Dimensional Electron Microscopy, Gordon Research Conference
    • Place of Presentation
      Hong Kong, China
    • Year and Date
      2016-06-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] エボラウイルスNP helixのクライオ電子顕微鏡解析2016

    • Author(s)
      杉田 征彦
    • Organizer
      Fifth Negative Strand Virus-Japan Symposium
    • Place of Presentation
      沖縄
    • Year and Date
      2016-01-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] Cryo-electron microscopic analysis of influenza virus ribonucleoprotein complex during transcription2015

    • Author(s)
      Yukihiko Sugita
    • Organizer
      East Asia Joint Symposium 2015
    • Place of Presentation
      Okinawa
    • Year and Date
      2015-11-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cryo-electron microscopic analysis of Ebola viral nucleocapcid2015

    • Author(s)
      Yukihiko Sugita
    • Organizer
      4th Negative Strand Virus-Japan Symposium
    • Place of Presentation
      沖縄ラグナガーデンホテル
    • Year and Date
      2015-01-19 – 2015-01-21
    • Related Report
      2014 Annual Research Report

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Published: 2014-09-09   Modified: 2017-05-10  

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