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Viruses in the ocean as a gene resource: development of RNA virus surveillance method and assessment of their function

Research Project

Project/Area Number 26892031
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Applied molecular and cellular biology
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

URAYAMA Syunichi  国立研究開発法人海洋研究開発機構, 海洋生命理工学研究開発センター, ポストドクトラル研究員 (50736220)

Project Period (FY) 2014-08-29 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords海洋 / ウイルス / 遺伝資源 / RNA / dsRNA / メタゲノム
Outline of Final Research Achievements

A comprehensive dsRNA sequencing method was developed, and RNA virus community in the ocean was assessed. The method enabled us to detect total non-Retro RNA viruses in any cellular sample without host phenotypes. With this method, we detected more than 20 virus genomes, suggesting that unseen RNA viruses should also exist in the ocean.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • Research Products

    (11 results)

All 2016 2015 2014 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Book (2 results) Remarks (2 results)

  • [Journal Article] FLDS: A Comprehensive dsRNA Sequencing Method for Intracellular RNA Virus Surveillance2016

    • Author(s)
      Syun-ichi Urayama, Yoshihiro Takaki, Takuro Nunoura.
    • Journal Title

      Microbes and Environments

      Volume: 31 Issue: 1 Pages: 33-40

    • DOI

      10.1264/jsme2.ME15171

    • NAID

      130005138799

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A New Fractionation and Recovery Method of Viral Genomes Based on Nucleic Acid Composition and Structure Using Tandem Column Chromatography2015

    • Author(s)
      Urayama S, Yoshida-Takashima Y, Yoshida M, Tomaru Y, Moriyama H, Takai K, Nunoura T
    • Journal Title

      Microbes and Environments

      Volume: 30 Issue: 2 Pages: 199-203

    • DOI

      10.1264/jsme2.ME14174

    • NAID

      130005077995

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 宿主病徴に依存しない網羅的RNAウイルスゲノム決定手法の確立2016

    • Author(s)
      浦山俊一、吉田ゆかり、高木善弘、高井研、布浦拓郎
    • Organizer
      第10回日本ゲノム微生物学会年会
    • Place of Presentation
      東京工業大学 大岡山キャンパス(東京都目黒区)
    • Year and Date
      2016-03-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] 高効率dsRNAシーケンシングによる細胞内RNAウイルスワールドの発見2015

    • Author(s)
      浦山俊一
    • Organizer
      日本微生物生態学会第30回大会
    • Place of Presentation
      土浦亀城プラザ(茨城県土浦市)
    • Year and Date
      2015-10-20
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Fractionation of four types of viral nucleic acids by using hydroxyapatite and cellulose chromatography2015

    • Author(s)
      Syun-ichi Urayama, Yukari Yoshida, Mitsuhiro Yoshida, Ken Takai, Takuro Nunoura
    • Organizer
      The annual meeting of the American Society for Virology
    • Place of Presentation
      Western University London ON, Canada
    • Year and Date
      2015-07-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ウイルス核酸種4種の同時分画及びdsRNAメタゲノム手法の確立2015

    • Author(s)
      浦山俊一、吉田(高島)ゆかり、吉田光宏、高木善弘、高井研、布浦拓郎
    • Organizer
      日本ゲノム微生物学会
    • Place of Presentation
      神戸大学農学部、兵庫県神戸市
    • Year and Date
      2015-03-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] いもち病菌弱毒化ウイルス研究が拓くウイルスの遺伝資源利用―ヴァイロコントロール研究者、海で溺れる?―2014

    • Author(s)
      浦山俊一
    • Organizer
      日本植物病理学会 関東部会
    • Place of Presentation
      宇都宮大学農学部、栃木県宇都宮市
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Book] バイオサイエンスとインダストリー2016

    • Author(s)
      浦山俊一・布浦拓郎.
    • Total Pages
      2
    • Publisher
      財団法人バイオインダストリー協会
    • Related Report
      2015 Annual Research Report
  • [Book] 生物の科学 遺伝2015

    • Author(s)
      浦山俊一・吉田光宏・吉田(高島)ゆかり.
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2015 Annual Research Report
  • [Remarks] JAMSTEC研究者総覧

    • URL

      http://www.jamstec.go.jp/souran/html/Shunichi_Urayama003613-j.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 浦山俊一 - 略歴 - jamstec

    • URL

      http://www.jamstec.go.jp/rcmb/j/member/urayama_syunichi.html

    • Related Report
      2014 Annual Research Report

URL: 

Published: 2014-09-09   Modified: 2017-05-10  

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