Development of a new methodology to produce seedlings for stock enhancement: Production of seedlings derived from cryopreserved germ cells of wild tiger puffer
| Project/Area Number |
26892033
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Aquatic bioproduction science
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| Research Institution | National Fisheries University |
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Principal Investigator |
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | トラフグ / 生殖幹細胞 / 凍結保存 / 遺伝資源 / 種苗放流 / 栽培漁業 / 代理親魚技術 / 生殖細胞移植 / 生殖細胞 / 遺伝的多様性 / 発生工学 |
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| Outline of Final Research Achievements |
This study established a methodology for cryopreservation of whole testes collected from tiger puffer in order to utilize genetic resources of captive wild fish into seed production for stock enhancement. By transplanting cryopreserved testicular cells contained spermatogonial stem cells (SSCs) of tiger puffer, the transplanted SSCs resumed spermatogenesis in grass puffer as surrogate recipient, and ultimately differentiated into functional sperm within the recipient gonads.
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Report
(3 results)
Research Products
(1 results)
