| Project/Area Number |
26893007
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 造血幹細胞 / 放射線 / 網羅的遺伝子解析 / 放射線感受性 / 造血幹/前駆細胞 / 遺伝子 / MYC |
|---|
| Outline of Final Research Achievements |
To clarify the nature of genes that contribute to the radiosensitivity of human hematopoietic stem/progenitor cells (HSPCs), we analyzed the gene expression profiles detected in HSPCs irradiated with 2 Gy X-rays after culture with or without an optimal combination of hematopoietic cytokines. A comprehensive genetic analysis to search for genes associated with cellular radiosensitivity was undertaken, and we found that the expressions of the downstream genes of MYC oncogene increased after X-irradiation. In fact, the activation of MYC was observed immediately after X-irradiation, and was the only gene still showing activation at 6 h after irradiation. Activated gene regulator function of MYC due to irradiation was suppressed by culturing the HSPCs with combinations of cytokines, which exerted radioprotective effects. MYC was strongly associated with the radiosensitivity of HSPCs.
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