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Expression analyzing of radiation responsive genes in human hematopoietic stem/progenitor cells

Research Project

Project/Area Number 26893007
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Radiation science
Research InstitutionHirosaki University

Principal Investigator

Tsujiguchi Takakiyo  弘前大学, 保健学研究科, 助手 (90737454)

Project Period (FY) 2014-08-29 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords造血幹細胞 / 放射線 / 網羅的遺伝子解析 / 放射線感受性 / 造血幹/前駆細胞 / 遺伝子 / MYC
Outline of Final Research Achievements

To clarify the nature of genes that contribute to the radiosensitivity of human hematopoietic stem/progenitor cells (HSPCs), we analyzed the gene expression profiles detected in HSPCs irradiated with 2 Gy X-rays after culture with or without an optimal combination of hematopoietic cytokines. A comprehensive genetic analysis to search for genes associated with cellular radiosensitivity was undertaken, and we found that the expressions of the downstream genes of MYC oncogene increased after X-irradiation. In fact, the activation of MYC was observed immediately after X-irradiation, and was the only gene still showing activation at 6 h after irradiation. Activated gene regulator function of MYC due to irradiation was suppressed by culturing the HSPCs with combinations of cytokines, which exerted radioprotective effects. MYC was strongly associated with the radiosensitivity of HSPCs.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • Research Products

    (8 results)

All 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (7 results) (of which Int'l Joint Research: 5 results)

  • [Journal Article] Expression analysis of radiation-responsive genes in human hematopoietic stem/progenitor cells.2016

    • Author(s)
      T. Tsujiguchi , T. Hirouchi , S. Monzen , Y. Tabuchi , I. Takasaki , T. Kondo , I. Kashiwakura.
    • Journal Title

      Journal of Radiation Research

      Volume: 57(1) Issue: 1 Pages: 35-43

    • DOI

      10.1093/jrr/rrv071

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Thrombopoietin-Mimetic Romiplostim Confers the Complete Survival Rate to Mice Exposed to Lethal Ionizing Radiation.2015

    • Author(s)
      Masaru Yamaguchi, Tokuhisa Hirouchi, Mitsuru Chiba, Satoru Monzen, Hironori Yoshino, Junya Ishikawa, Takakiyo Tsujiguchi, Ayaka Nishiyama, Sho Murakami, Junichiro Komura, Ikuo Kashiwakura.
    • Organizer
      ASH2015
    • Place of Presentation
      Orland, USA
    • Year and Date
      2015-12-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] EXPRESSION ANALYSIS OF C-MYC IN BONE MARROW CELLS OF MICE EXPOSED TO IONIZING RADIATION.2015

    • Author(s)
      K Yokoyama, A Nishiyama, T Tsujiguchi, S Murakami, M Yamaguchi, I Kashiwakura
    • Organizer
      2nd Educational Symposium on Radiation and Health by young scientists
    • Place of Presentation
      弘前大学保健学研究科(青森県, 弘前市)
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of a c-Mpl Agonist on Mice Exposed to Lethal Ionizing Radiation.2015

    • Author(s)
      M Yamaguchi, T Hirouchi, M Chiba, S Monzen, H Yoshino, J Ishikawa, T Tsujiguchi, A Nishiyama, S Murakami, J Komura and I Kashiwakura
    • Organizer
      2nd Educational Symposium on Radiation and Health by young scientists
    • Place of Presentation
      弘前大学保健学研究科(青森県, 弘前市)
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 電離放射線暴露マウス骨髄有核細胞におけるMyc遺伝子発現の解析.2014

    • Author(s)
      西山 彩香 辻口 貴清 山口 平 村上 翔 柏倉 幾郎
    • Organizer
      日本放射線影響学会第57回大会
    • Place of Presentation
      かごしま県民交流センター(鹿児島県, 鹿児島市)
    • Year and Date
      2014-10-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] 致死放射線ばく露マウスに対するc-Mpl作動薬の作用2014

    • Author(s)
      山口 平 廣内 篤久 石川 純也 辻口 貴清 西山 彩香 村上 翔 小村 潤一郎 柏倉 幾郎
    • Organizer
      日本放射線影響学会第57回大会
    • Place of Presentation
      かごしま県民交流センター(鹿児島県, 鹿児島市)
    • Year and Date
      2014-10-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] Expression analysis of radiation responsive genes in human hematopoietic stem/progenitor cells.2014

    • Author(s)
      Takakiyo Tsujiguchi, Tokuhisa Hirouchi, Satoru Monzen, Yoshiaki Tabuchi, Ichiro Takasaki, Takashi Kondo and Ikuo Kashiwakura
    • Organizer
      1st Educational Symposium on Radiation and Health by young scientists
    • Place of Presentation
      ホテルニューキャッスル(青森県, 弘前市)
    • Year and Date
      2014-09-22
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of a c-mpl receptor agonist on mice exposed to lethal ionizing radiation.2014

    • Author(s)
      M. Yamaguchi, T. Hirouchi, M. Chiba, S. Monzen, H. Yoshino, J. Ishikawa, T. Tsujiguchi, A. Nishiyama, S. Murakami, J. Komura, I. Kashiwakura
    • Organizer
      The 9th International Symposium on the Natural Radiation Environment
    • Place of Presentation
      ホテルニューキャッスル(青森県, 弘前市)
    • Year and Date
      2014-09-22
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research

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Published: 2014-09-09   Modified: 2017-05-10  

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