| Project/Area Number |
26893031
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Endocrinology
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| Research Institution | Gunma University |
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Principal Investigator |
KIKUCHI Osamu 群馬大学, 生体調節研究所, 研究員 (40739009)
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| Project Period (FY) |
2014-08-29 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | グルカゴン / Sirt1 |
|---|
| Outline of Final Research Achievements |
We hypothesized that “Sirt1 up-regulates cell differentiation, proliferation and neogenesis, and proglucagon gene expression via activation of FoxO1 in α cells”. To investigate this hypothesis, we analyzed phenotype of the α cell-specific Sirt1 gene knockout (KO) mice.
The KO mice showed a significant suppression of recovery from insulin induced hypoglycemia compared with the control mice. This result suggests glucagon secretion was impaired in the KO mice. Moreover, we validated a Sirt1 inhibitor suppressed glucagon secretion and an activator enhanced it by in vitro analysis used α cell line. Both results supported the hypothesis.
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