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Localization analysis of synovial mesenchymal stem cells aimed for the technological development of cartilage and meniscus regenerative medicine

Research Project

Project/Area Number 26893066
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Mizuno Mitsuru  東京医科歯科大学, 再生医療研究センター, 特任研究員 (00733908)

Project Period (FY) 2014-08-29 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords再生医療 / 滑膜幹細胞 / 変形性膝関節症 / 滑膜組織 / 半月板 / 関節軟骨 / 再生医学 / 間葉系幹細胞 / 局在解析 / 軟骨再生 / 再生医療技術
Outline of Final Research Achievements

In Japan, which has become a super-aged society, to develop a superior regenerative medical technology for meniscal damage and cartilage degeneration associated with knee osteoarthritis is awaited by huge number of the patients estimated to be about 8.5 million. In this research, we tried to develop basic technologies based on the characteristics by performing identification to the localization of synovial mesenchymal stem cells and functional analysis of purified cells. We have suggested the localization of the synovial stem cells and presented the results at the 15th The Japanese Society for Regenerative Medicine. In addition, we have been developed stem cell culture methods for clinical cell transplantation study using human serum, which was instability and high safety material, by the functional analysis of purified cells. Then, we have published its research achievements in “Stem cell research and therapy” that was peer reviewed in English.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • Research Products

    (12 results)

All 2016 2015

All Journal Article (6 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 6 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Cartilage derived from bone marrow mesenchymal stem cells expresses lubricin in vitro and in vivo2016

    • Author(s)
      Yusuke Nakagawa, Takeshi Muneta, Koji Otabe, Nobutake Ozeki, Mitsuru Mizuno, Mio Udo, Ryusuke Saito, Katsuaki Yanagisawa, Shizuko Ichinose, Hideyuki Koga, Kunikazu Tsuji and Ichiro Sekiya
    • Journal Title

      PLoS ONE

      Volume: 11 Issue: 2 Pages: e0148777-e0148777

    • DOI

      10.1371/journal.pone.0148777

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Not single but periodic injections of synovial mesenchymal stem cells maintain viable cells in knees and inhibit osteoarthritis progression in rats.2016

    • Author(s)
      Ozeki N, Muneta T, Koga H, Nakagawa Y, Mizuno M, Tsuji K, Mabuchi Y, Akazawa C, Kobayashi E, Matsumoto K, Futamura K, Saito T, Sekiya I
    • Journal Title

      Osteoarthritis Cartilage

      Volume: in press Issue: 6 Pages: 1061-70

    • DOI

      10.1016/j.joca.2015.12.018

    • NAID

      40021330438

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transplantation of autologous synovial mesenchymal stem cells promotes meniscus regeneration in aged primates.2016

    • Author(s)
      Kondo S, Muneta T, Nakagawa Y, Koga H, Watanabe T, Tsuji K, Sotome S, Okawa A, Kiuchi S, Ono H, Mizuno M, Sekiya I
    • Journal Title

      J Orthop Res

      Volume: in press Issue: 6 Pages: 1274-1282

    • DOI

      10.1002/jor.23211

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Platelet-derived growth factor (PDGF)-AA/AB in human serum are potential indicators of the proliferative capacity of human synovial mesenchymal stem cells.2015

    • Author(s)
      Mizuno M, Katano H, Otabe K, Komori K, Matsumoto Y, Fujii S, Ozeki N, Tsuji K, Koga H, Muneta T, Matsuyama A, Sekiya
    • Journal Title

      Stem Cell Res Ther.

      Volume: 10 Issue: 1 Pages: 243-243

    • DOI

      10.1186/s13287-015-0239-2

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Synovial mesenchymal stem cells promote healing after meniscal repair in microminipigs.2015

    • Author(s)
      Nakagawa Y, Muneta T, Kondo S, Mizuno M, Takakuda K, Ichinose S, Tabuchi T, Koga H, Tsuji K, Sekiya I.
    • Journal Title

      Osteoarthritis Cartilage.

      Volume: in press Issue: 6 Pages: 1007-1017

    • DOI

      10.1016/j.joca.2015.02.008

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Synovial mesenchymal stem cells promote meniscus regeneration augmented by an autologous Achilles tendon graft in a rat partial meniscus defect model2015

    • Author(s)
      N. Ozeki, T. Muneta, S. Matsuta, M. Mizuno, et al.
    • Journal Title

      Stem Cells

      Volume: in press

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] PDGF-AA/AB in human serum are potential indicators of the proliferative capacity of human synovial mesenchymal stem cells2016

    • Author(s)
      Mitsutu Mizuno, Hisako Katano, Koji Otabe, Keiiciro Komori, Yukie Matsumoto, Nobutake Ozeki, Kunikazu Tsuji, Takeshi Muneta, Ichiro Sekiya
    • Organizer
      Orthopaedic Research Society 2016 Annual Meeting
    • Place of Presentation
      Orlando, Florida (USA)
    • Year and Date
      2016-03-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Properties of MSCs derived from Surface, Stroma, and Perivascular synovium of OA patients2016

    • Author(s)
      Mitsutu Mizuno, Hisako Katano, Yo Mabuchi, Yusuke Ogata, Shizuko Ichinose, Koji Otabe, Keiiciro Komori, Yukie Matsumoto, Nobutake Ozeki, Kunikazu Tsuji, Chiro Akazawa, Takeshi Muneta, Ichiro Sekiya
    • Organizer
      Orthopaedic Research Society 2016 Annual Meeting
    • Place of Presentation
      Orlando, Florida (USA)
    • Year and Date
      2016-03-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 緩徐に凝集させ分離したヒト血清はPDGF-AA濃度が増し滑膜幹細胞の増殖を強める2015

    • Author(s)
      水野 満、宗田 大、小森 啓一郎、片野 尚子、松本 幸恵、小田邉 浩二、辻 邦和、大川 淳、関矢 一郎
    • Organizer
      第30回日本整形外科学会基礎学術集会
    • Place of Presentation
      富山国際会議場ほか(富山市, 富山)
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 滑膜幹細胞を用いたヒト血清の増殖性を予測する因子の探索2015

    • Author(s)
      水野 満
    • Organizer
      第36回日本炎症・再生医学会
    • Place of Presentation
      虎ノ門ヒルズフォーラム(東京)
    • Year and Date
      2015-07-21 – 2015-07-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 滑膜幹細胞の増殖を促進するヒト血清分離法の工夫2015

    • Author(s)
      水野 満、小森 啓一郎、片野 尚子、松本 幸恵、小田邉 浩二、 関矢 一郎
    • Organizer
      第36回日本炎症・再生医学会
    • Place of Presentation
      虎ノ門ヒルズフォーラム(港区, 東京)
    • Year and Date
      2015-07-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] 滑膜幹細胞増殖を予測する自己血清評価法の確立2015

    • Author(s)
      水野 満
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Annual Research Report

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Published: 2014-09-09   Modified: 2017-05-10  

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