| Project/Area Number |
26893112
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | エピジェネティック制御 / epigenetics / hormone receptor / 卵胞発育制御 / 顆粒膜細胞 |
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| Outline of Final Research Achievements |
The normal growth of the granulosa cells is essential for obtaining a high-quality egg with a high potential to be fertilized. Granulosa cells, which support the growth of eggs, begins and increases its expression of the luteinizing hormone receptor (LHR) as the ovarian follicle grows. Here we studied the epigenetic regulation of LHR expression. Epigenetics is the regulation of gene expression which do not involve changes in the underlying DNA sequence. Such regulations include histone modification and DNA methylation. In this study, we studied histone modifications and DNA methylations of the LHR promoter region. Our results show that histone acetylation of H4K8 and H3K9, and DNA metylation of the 2nd,4th 5th and 9th CpG sites are important in elevating LHR expression.
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