The effect of dietary amino acids balance for mouse organs under caloric restriction.

• Project/Area Number: 26893116
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General internal medicine(including psychosomatic medicine)
• Research Institution: Shiga University of Medical Science
• Principal Investigator: YAMAHARA KOSUKE 滋賀医科大学, 医学部, 客員助教 (50731915)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 加齢 / 栄養学 / アミノ酸 / 老化

Outline of Final Research Achievements

It has been reported that caloric restriction protects cell damages and prolongs life span. We conducted mouse experiment which is a diet restriction model by using the special diet which is modified the balance of amino acids. Life span and kidney function were improved in caloric restriction group compared to the ad libitum mice group, however these effects were canceled in essential amino acids-rich diet group. Amino acid modification diet may induce effects on life span and organ functions of mice.
To reveal the effects of dietary amino acids in other organs, we have conducted new experiments using diet restriction with amino acid modification. We analyzed glucose tolerance, muscle power and cardiac function. Caloric restriction improved the glucose tolerance, cardiac ejection fraction. On the other hand, muscle power was decreased in caloric restriction group. Amino acids rich diet improves muscle power induced by caloric restriction, however glucose tolerance tend to worsen.

Research Products

• [Journal Article] Impaired podocyte autophagy exacerbates proteinuria in diabetic nephropathy. (2016)
  • Author(s): Tagawa A, Yasuda M, Kume S, Ymahara K, Nakazawa J, Chin-Kanasaki M, Araki H, Araki SI, Koya D, Asanuma K, Kim EH, Haneda M, Kajiwara N, Hayashi K, Ohashi H, Ugi S, Maegawa H, Uzu T
  • Journal Title: Diabetes. Volume: - Issue: 3 Pages: 755-767
  • DOI: 10.2337/db15-0473
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Lamp-2 deficiency prevents high-fat diet-induced obese diabetes via enhancing energy expenditure. (2015)
  • Author(s): Yasuda-Yamahara M, Kume S, Yamahara K, Nakazawa J, Chin-Kanasaki M, Araki H, Araki S, Koya D, Haneda M, Ugi S, Maegawa H, Uzu T.
  • Journal Title: Biochem Biophys Res Commun. Volume: 18 Issue: 2 Pages: 249-255
  • DOI: 10.1016/j.bbrc.2015.08.010
  • Peer Reviewed / Int'l Joint Research
• [Book] 糖尿病性腎症克服に残された課題～挑戦 発症メカニズムに立脚した治療の将来展望 (2015)
  • Author(s): 久米真司、安田真子、山原康佑
  • Total Pages: 7
  • Publisher: 月刊糖尿病