Investigation of the regulatory mechanism of perinatal cardiomyocyte maturation

• Project/Area Number: 26893124
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Cardiovascular medicine
• Research Institution: Kyoto University
• Principal Investigator: NAKASHIMA YASUHIRO 京都大学, 医学(系)研究科(研究院), 助教 (20565585)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 心筋分化 / 心発生 / 細胞増殖 / 心筋細胞増殖

Outline of Final Research Achievements

Proliferative capacity of adult mammalian cardiomyocytes is very limited. However, its regulatory mechanism is still unclear. Since the recent studies have suggested that the cardiomyocytes soon after birth retain the proliferative capacity to repair damaged cardiac tissue in vivo, we searched possible regulatory factors of cardiomyocyte proliferation among the genes expressed dominantly in perinatal mouse heart and identified several genes that are expressed dominantly in the perinatal stage and also promote proliferation in H9C2 cells by using immunohistochemistry of mouse heart tissue, gene knockdown and overexpression in vitro. Next we investigate the molecular mechanism by which these genes regulate proliferation of the cells including cardiomyocytes.

Research Products

• [Journal Article] Expression Patterns of miRNA-423-5p in the Serum and Pericardial Fluid in Patients Undergoing Cardiac Surgery. (2015)
  • Author(s): Miyamoto S, Usami S, Kuwabara Y, Horie T, Baba O, Hakuno D, Nakashima Y, Nishiga M, Izuhara M, Nakao T, Nishino T, Ide Y, Nakazeki F, Wang J, Ueyama K, Kimura T, Ono K.
  • Journal Title: PLoS One. Volume: 10(11) Issue: 11 Pages: e0142904-e0142904
  • DOI: 10.1371/journal.pone.0142904
  • NAID: 120005749912
  • Peer Reviewed / Open Access
• [Journal Article] Heterozygous deletion of sarcolipin maintains normal cardiac function. (2015)
  • Author(s): Shimura D, Kusakari Y, Sasano T, Nakashima Y, Nakai G, Jiao Q, Jin M, Yokota T, Ishikawa Y, Nakano A, Goda N, Minamisawa S.
  • Journal Title: Am J Physiol Heart Circ Physiol. Volume: 310(1) Issue: 1 Pages: 92-103
  • DOI: 10.1152/ajpheart.00411.2015
  • Peer Reviewed / Open Access / Int'l Joint Research