| Project/Area Number |
26893136
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Osaka University |
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Principal Investigator |
Hiyama Satoshi 大阪大学, 医学(系)研究科(研究院), 寄附講座助教 (10735335)
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| Co-Investigator(Kenkyū-buntansha) |
TUJII MASAHIKO 東大阪市立総合病院, 院長 (40303937)
IIJIMA HIDEKI 大阪大学, 医学系研究科, 講師 (90444520)
SHINZAKI SHINICHIRO 大阪大学, 医学系研究科, 助教 (60546860)
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| Co-Investigator(Renkei-kenkyūsha) |
IIDA TETUYA 大阪大学, 微生物病研究所, 教授 (90221746)
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | パイエル板 / 腸管免疫 / 腸内細菌 / 炎症性腸疾患 |
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| Outline of Final Research Achievements |
Lymphoid tissue was resected from Peyer’s patches (PP) of the wild-type mice using laser microdissection method (LMD). When we analyzed the number of bacteria from the extracted DNA from PP and heart (negative control) using LMD, the number of bacteria involved in PP had higher tendency compared with those from heart. This indicates that detectable number of bacteria inhabits inside of PP.
Moreover, the profiles of bacteria identified from PP, heart and feces samples was evaluated using metagenomics analysis, which revealed that each group showed distinct profiles. Although potential contamination cannot be denied completely, this data suggests the possibility that specific bacteria species might exist inside of PP.
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