Analysis of osteoblast differentiation mechanism using a Id2-deficient mice derived from iPS cells

• Project/Area Number: 26893143
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Osaka University
• Principal Investigator: Uraguchi Shinya 大阪大学, 歯学部附属病院, 医員 (80737528)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: iPS細胞 / 骨芽細胞分化 / Id2 / Id2遺伝子

Outline of Final Research Achievements

iPS cells are expected to be useful for alveolar bone augmentation/regeneration in dental implant and prothodontic treatment; however the mechanisms of iPS cell osteogenesis remain unclear. The objective is to investigate the role of Id2 in iPS cell osteogenesis by establishing Id2-deficient model iPS cells. The induction of iPS cells from gingival fibroblasts of Id2-/- or Id2+/+ mice was performed by retroviral transduction. iPS cells induced osteogenic differentiation and analyzed by ALP staining etc. The Id2-/--iPS cells showed higher osteogenic gene expression than Id2+/+-iPS cells. We established an osteogenesis model with Id2-deficient iPS cells, which demonstrated enhanced osteogenic differentiation and represent an important step toward the further analysis of novel mechanisms underlying iPS cell osteogenesis.