Effect of trophic factors released from mesenchymal stem cells on the periodontal tissue

• Project/Area Number: 26893144
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Periodontology
• Research Institution: Osaka University
• Principal Investigator: Sawada Keigo 大阪大学, 歯学部附属病院, 医員 (70733054)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 歯学 / 再生医学 / 間葉系幹細胞 / 歯周炎 / Trophic因子

Outline of Final Research Achievements

In this study, trophic factors released by mesenchymal stem cells were examined for their paracrine effect on human periodontal tissue-forming cells.
Adipose tissue-derived mesenchymal stem cell (ADSC) conditioned medium stimulates the differentiation of human periodontal ligament cell (HPDL) to mineralized tissue-forming cells. Besides, ADSC secreted insulin-like growth factor binding protein 6 (IGFBP6). Furthermore, the positive effects of ADSC conditioned medium on HPDL differentiation were significantly suppressed by transfecting ADSCs with IGFBP6 siRNA. These results revealed the mechanism that ADSC transplanted into the periodontal tissue release trophic factors that can stimulate the differentiation of HPDLs to mineralized tissue-forming cells.

Research Products

• [Presentation] The mechanism of periodontal tissue regeneration by transplantation of adipose tissue-derived multi-lineage progenitor cells. (2016)
  • Author(s): Keigo Sawada, Masahide Takedachi, Tomoaki Iwayama, Satomi Yamamoto, Chiaki Morimoto, Asae Hirai, Chun Man Lee, Hanayuki Okura, Akifumi Matsuyama, Yuko Sano, Masahiro Kitamura, Shinya Murakami.
  • Organizer: ISSCR 2016
  • Place of Presentation: Moscone Center West, San Francisco, USA
  • Year and Date: 2016-06-22
  • Int'l Joint Research
• [Presentation] ADMPC移植による歯周組織再生誘導効果とその作用機序の検討 (2016)
  • Author(s): 沢田啓吾、竹立匡秀、岩山智明、山本智美、森本千晶、平井麻絵、李千萬、松山晃文、大倉華雪、佐野夕子、北村正博、村上伸也
  • Organizer: 第59回春季日本歯周病学会
  • Place of Presentation: かごしま県民交流センター、鹿児島市、鹿児島県
  • Year and Date: 2016-05-20
• [Presentation] 脂肪組織由来多系統前駆細胞の移植による新規歯周組織再生療法の開発 (2016)
  • Author(s): 沢田啓吾、竹立匡秀、岩山智明、山本智美、森本千晶、平井麻絵、李千萬、松山晃文、大倉華雪、佐野夕子、野崎剛徳、北村正博、村上伸也
  • Organizer: 第15回日本再生医療学会総会
  • Place of Presentation: 大阪国際会議場、大阪市、大阪府
  • Year and Date: 2016-03-17