metabolic analysis of therapy resistant cancer stem cell

• Project/Area Number: 26893172
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Yamaguchi University
• Principal Investigator: Nishikawa Shimpei 山口大学, 共同獣医学部, 助教 (90730565)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 間葉系幹細胞 / 癌幹細胞 / マーカー / ALDH / 脂肪由来幹細胞 / アルデヒド脱水素酵素 / 代謝 / 分化能 / 組織幹細胞

Outline of Final Research Achievements

To characterize cancer stem cell and normal tissue stem cell, we investigated marker expression in normal mouse adipose-derived stem cell. As a screening result of analysis for surface markers, phosphorylation-specific antibodies, and aldehyde dehydrogenase activity (ALDH), small subpopulation with high ALDH activity (ALDHhi cells) was found. ALDHhi cells showed higher ability to differentiate to ostogenic lineage. Further analysis on gene expression using microarray and gene expression enrichment analysis is in progress.