| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
We, for the first time, could subdivide granulocyte/macrophage progenitor (GMP) population based on the expression pattern of Vcam-1 and M-CSFR: a fraction (10-20%) of GMPs expressed high level of Vcam-1. We found Vcam-1hiM-CSFR- GMPs, both on a single cell level and as a population, robustly generated neutrophils but no other lineages in vitro. In vivo transplantation assays also showed that Vcam-1hiM-CSFR- GMPs completely lacked monocyte/macrophage producing potential. Thus, we termed Vcam-1hiM-CSFR- GMPs as the NPs. Gene expression analyses revealed that Vcam-1hiM-CSFR- GMPs upregulated neutrophil-related genes (e.g., Gfi-1, G-CSFR) and downregulated monocyte/macrophage-related genes (e.g., Irf8, Klf4), clearly reflecting their differentiation potential. This newly classified population might be a useful tool for understanding the molecular mechanisms of physiological/pathological neutrophil development.
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