Identification of neutrophil lineage-committed progenitors and their developmental mechanism
| Project/Area Number |
26893186
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Kyushu University |
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Principal Investigator |
Mori Yasuo 九州大学, 大学病院, 助教 (90573345)
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| Research Collaborator |
MIYAWAKI Kohta
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 好中球 / 前駆細胞 / 分化 / 系統決定 / 転写因子 |
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| Outline of Final Research Achievements |
We, for the first time, could subdivide granulocyte/macrophage progenitor (GMP) population based on the expression pattern of Vcam-1 and M-CSFR: a fraction (10-20%) of GMPs expressed high level of Vcam-1. We found Vcam-1hiM-CSFR- GMPs, both on a single cell level and as a population, robustly generated neutrophils but no other lineages in vitro. In vivo transplantation assays also showed that Vcam-1hiM-CSFR- GMPs completely lacked monocyte/macrophage producing potential. Thus, we termed Vcam-1hiM-CSFR- GMPs as the NPs. Gene expression analyses revealed that Vcam-1hiM-CSFR- GMPs upregulated neutrophil-related genes (e.g., Gfi-1, G-CSFR) and downregulated monocyte/macrophage-related genes (e.g., Irf8, Klf4), clearly reflecting their differentiation potential. This newly classified population might be a useful tool for understanding the molecular mechanisms of physiological/pathological neutrophil development.
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Report
(3 results)
Research Products
(1 results)
