| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Hepatic resection and liver transplantation are considered to be curative treatments for HCC. Despite other forms of effective therapies including local ablation therapy and transcatheter arterial chemoembolization, many patients show local recurrence and finally progress to the advanced stages with vascular invasion and multiple intrahepatic metastases. In this study, we aimed to investigate the role of micro-RNA (miRNA) on the progression for local recurrence in HCC.
We generated sphere formational Huh7 cells. Sphere formational Huh7 cells exhibited lower proliferation than control cells. There was higher tolerance for reactive oxygen species with sphere formational Huh7 cells as compared with control cells. We then used a miRNA microarray analysis to investigate the epigenetics change induced by sphere formation. An up-regulated miRNA was confirmed by real time PCR analysis in clinical HCC samples.
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