Role of micro-RNA on the progression for local recurrence in hepatocellular carcinoma

• Project/Area Number: 26893191
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Kyushu University
• Principal Investigator: ITOH Shinji 九州大学, 大学病院, 助教 (90382423)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 肝細胞癌 / マイクロRNA / スフェア

Outline of Final Research Achievements

Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Hepatic resection and liver transplantation are considered to be curative treatments for HCC. Despite other forms of effective therapies including local ablation therapy and transcatheter arterial chemoembolization, many patients show local recurrence and finally progress to the advanced stages with vascular invasion and multiple intrahepatic metastases. In this study, we aimed to investigate the role of micro-RNA (miRNA) on the progression for local recurrence in HCC.
We generated sphere formational Huh7 cells. Sphere formational Huh7 cells exhibited lower proliferation than control cells. There was higher tolerance for reactive oxygen species with sphere formational Huh7 cells as compared with control cells. We then used a miRNA microarray analysis to investigate the epigenetics change induced by sphere formation. An up-regulated miRNA was confirmed by real time PCR analysis in clinical HCC samples.

Research Products

• [Journal Article] Relevance of microRNA-18a and microRNA-199a-5p to hepatocellular carcinoma recurrence after living donor liver transplantation (2016)
  • Author(s): Morita K, Shirabe K, Taketomi A, Soejima Y, Yoshizumi T, Uchiyama H, Ikegami T, Yamashita YI, Sugimachi K, Harimoto N, Itoh S, Ikeda T, Maehara Y
  • Journal Title: Liver Transplantation Volume: 22 Issue: 5 Pages: 665-676
  • DOI: 10.1002/lt.24400
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 肝細胞癌におけるEphB2発現の生物学的意義 (2015)
  • Author(s): 伊藤心二、下川雅弘、坂田一仁、冨野高広、吉田佳弘、別城悠樹、王 歓林、今井大祐、木村光一、岡部弘尚、播本憲史、池上 徹、内山秀昭、吉住朋晴、池田哲夫、調 憲、 前原喜彦
  • Organizer: 第26回日本消化器癌発生学会総会
  • Place of Presentation: 米子
  • Year and Date: 2015-11-19
• [Presentation] 肝細胞癌におけるEphB2発現の生物学的意義 (2015)
  • Author(s): 伊藤心二、調 憲、今井大祐、前原喜彦
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08