Somatic cell fate conversion by reprogramming factor isolated from Lactic acid bacteria

• Project/Area Number: 26893200
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kumamoto University
• Principal Investigator: Ito Naofumi 熊本大学, 大学院生命科学研究部(医), 研究員 (60732716)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 発生 / 細胞分化 / 初期化 / リボソーム / 多能性幹細胞 / 細胞多能性 / リプログラミング / 微生物 / リボゾーム

Outline of Final Research Achievements

In previous studies, by adding lactic acid bacterium cells to human dermal fibroblasts induced characteristic cell mass formation was revealed. The cell mass had a multiple differential ability beyond germ layers limit. Chromatographic fractionated lactic acid bacteria lysate was added to the human skin cells result the cell mass formation and identified high activity condition for the cell mass formation. The fraction was analyzed by mass spectrometer and identified few proteins. It was also found that the molecule of interest is a molecule of above 100 kDa by other biochemical analysis. It was identified that the molecule of interest is a ribosome.

Research Products

• [Journal Article] Reprogramming of human somatic cells by bacteria (2015)
  • Author(s): Naofumi Ito, Kunimasa Ohta
  • Journal Title: Dev. Growth Differ. Volume: 57 Issue: 4 Pages: 305-312
  • DOI: 10.1111/dgd.12209
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Somatic cell fate conversion by reprogramming factor isolated from Lactic acid bacteria. (2015)
  • Author(s): 伊藤 尚文
  • Organizer: 第5回 オルソオルガノジェネシス研究会議
  • Place of Presentation: 安曇野穂高ビューホテル (長野県安曇野市)
  • Year and Date: 2015-08-06
• [Presentation] Human cell reprogramming by defined factors isolated from acid bacteria. (2015)
  • Author(s): 伊藤 尚文
  • Organizer: 第48回日本発生生物学会大会（APDBN共催）
  • Place of Presentation: つくば国際会議場 ( 茨城県つくば市 )
  • Year and Date: 2015-06-03
• [Presentation] 細胞外分泌タンパク質Tsukushiは神経および血管系から発現される ことで脳神経幹細胞ニッチの制御に関与する (2014)
  • Author(s): 伊藤尚文
  • Organizer: 第37回日本分子生物学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25
• [Presentation] 細胞外分泌タンパク質Tsukushiは神経および血管系から発現される ことで脳神経幹細胞ニッチの制御に関与する (2014)
  • Author(s): 伊藤 尚文
  • Organizer: 熊本医学・生物科学国際シンポジウム
  • Place of Presentation: 熊本市医師会館 (熊本県熊本市)
  • Year and Date: 2014-09-04
• [Remarks] 熊本大学 大学院生命科学研究部 神経分化学教室
  • URL: http://srv02.medic.kumamoto-u.ac.jp/dept/devneuro/