| Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Accumulation of nonvolatile acid that taken from food causes metabolic acidosis. Acid is excreted into urine by the intercalated cells of the collecting duct of the kidney. Adrenocortical hormone, aldosterone, is a key molecule that regulates acid excretion in the intercalated cells. The effect of acidosis (low pH) on the intercalated cells is unclear. We applied transcriptional start site-sequencing to characterize low pH stimulated-genes in the IN-IC cells, a rat intercalated cell line. We found the increases of genes that involve in ubiquitin-proteasome-pathway. We confirmed by Western blot that ubiquitination of proteins is enhanced by low pH. Metabolic acidosis might regulate acid secretion in the intercalated cells by activating ubiquitin-proteasome-pathway.
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