Regulation mechanism of the expression of acid-base related genes in the intercalated cells of the collecting duct in the kidney
Project/Area Number |
26893202
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Kidney internal medicine
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Research Institution | Kumamoto University |
Principal Investigator |
Izumi Yuichiro 熊本大学, 医学部附属病院, その他 (20736243)
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Project Period (FY) |
2014-08-29 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | 腎集合尿細管 / 酸塩基平衡 / ユビキチン-プロテアソーム系 / ユビキチンープロテアソーム / アルドステロン |
Outline of Final Research Achievements |
Accumulation of nonvolatile acid that taken from food causes metabolic acidosis. Acid is excreted into urine by the intercalated cells of the collecting duct of the kidney. Adrenocortical hormone, aldosterone, is a key molecule that regulates acid excretion in the intercalated cells. The effect of acidosis (low pH) on the intercalated cells is unclear. We applied transcriptional start site-sequencing to characterize low pH stimulated-genes in the IN-IC cells, a rat intercalated cell line. We found the increases of genes that involve in ubiquitin-proteasome-pathway. We confirmed by Western blot that ubiquitination of proteins is enhanced by low pH. Metabolic acidosis might regulate acid secretion in the intercalated cells by activating ubiquitin-proteasome-pathway.
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Report
(3 results)
Research Products
(14 results)
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[Presentation] TSS-Seq analysis of low pH-induced gene transcription in the intercalated cells of the collecting ducts.2015
Author(s)
Izumi Y, Eguchi K, Nakagawa T, Nakayama Y, Inoue H, Kakizoe Y, Kuwabara T, Nonoguchi H, Mukoyama M.
Organizer
The American Society of Nephrology Kidney Week 2015
Place of Presentation
San Diego, CA, USA
Year and Date
2015-11-07
Related Report
Int'l Joint Research
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[Presentation] The protective role of doxycycline for cisplatin-induced AKI by its anti-inflammatory and anti-oxidative effects.2015
Author(s)
Nakagawa T, Kakizoe Y, Miyasato Y, Mizumoto T, Hayata M, Izumi Y, Kuwabara T, Miyoshi T, Adachi M, Kitamura K, Mukoyama M
Organizer
The American Society of Nephrology Kidney Week 2015
Place of Presentation
San Diego, CA, USA
Year and Date
2015-11-07
Related Report
Int'l Joint Research
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[Presentation] A case of proliferative glomerulonephritis with monoclonal IgG deposits exhibiting marked nephrotic syndrome who responded well to the renin-angiotensin system blockade alone.2015
Author(s)
Nishiguchi Y, Inoue H, Onoue T, Kakizoe Y, Izumi Y, Kuwabara T, Miyoshi T, Adachi M, Nakayama Y, Mukoyama M
Organizer
The American Society of Nephrology Kidney Week 2015
Place of Presentation
San Diego, CA, USA
Year and Date
2015-11-05
Related Report
Int'l Joint Research
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[Presentation] Erythropoietin production by the nephron.2014
Author(s)
Izumi Y, Yasuoka Y, Nagai T, Nakayama Y, Inoue H, Nakanishi T, Mukoyama M, Kawahara K, and Nonoguchi H.
Organizer
The American Society of Nephrology Kidney Week 2014
Place of Presentation
Philadelphia, PA
Year and Date
2014-11-15
Related Report