Development of cancer peptide vaccine that can induce tumor-specific CTLs and Th cells.

• Project/Area Number: 26893206
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Kumamoto University
• Principal Investigator: Yuno Akira 熊本大学, 医学部附属病院, 医員 (40738473)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: がんペプチドワクチン療法 / キラーT細胞 / ヘルパーT細胞 / 腫瘍免疫 / ペプチドワクチン療法 / 癌免疫療法 / がんワクチン研究

Outline of Final Research Achievements

Recently, it is reported the efficacy of cancer immunotherapy including cancer peptide vaccine. Many reports show cancer peptide vaccine can induce CTL. There are not only CTL but also Th cells in the T cell subset and they roll important function for eradication of tumor. So we tried to identify the epitope can induce both CTL and Th cells and conduct immunological analysis.

Research Products

• [Journal Article] Generation of Large Numbers of Antigen-Expressing Human Dendritic Cells Using CD14-ML Technology (2016)
  • Author(s): Imamura Y, Haruta M, Tomita Y, Matsumura K, Ikeda T, Yuno A, Hirayama M, Mizuta H, Nishimura Y, Senju S.
  • Journal Title: PLoS One. Volume: 11
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cancer immunotherapy using novel tumor-associated antigenic peptides identified by genome-wide cDNA microarray analyses (2015)
  • Author(s): Nishimura, Y., Tomita, Y., Yuno, A., Yoshitake, Y., Shinohara, M.
  • Journal Title: Cancer Science Volume: 106 Issue: 5 Pages: 505-511
  • DOI: 10.1111/cas.12650
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of Glypican-3-Derived Long Peptides Activating both CD8+ and CD4+ T-cells; Prolonged Overall Survival in Cancer Patients with Th Cell Response. (2015)
  • Author(s): Mohammad Abu Sayem, Tomita Y, Yuno A, Hirayama M, Irie A, Tsukamoto H, Senju S, Yuba E, Yoshikawa T, Kono K, Nakatsura T, Nishimura Y.
  • Journal Title: Oncoimmunology. Volume: 5
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Phase II clinical trial of multiple peptide vaccination for advanced head and neck cancer patients revealed induction of immune responses and improved OS. (2015)
  • Author(s): Yoshitake , Y., Fukuma, D., Yuno, A. , Hirayama, M. , Nakayama, H. , Tanaka. T., Nagata, M. , Takamune, Y., Kawahara, K., Nakagawa, Y., Yoshida, R., Hirosue, A., Ogi, H., Hiraki, A. , Jono, H., Hamada, A., Yoshida, K., Nishimura, Y., Nakamura, Y., and Shinohara, M
  • Journal Title: Clin. Cancer Res. Volume: 21 Pages: 312-321
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Identification of IMP-3-derived multiple HLA class II-restricted long peptides that can activate both CTLs and Th cells (2015)
  • Author(s): Hirayama Masatoshi, Tomita Yusuke, Yuno Akira, Mohammad Abu Sayem, Tsukamoto Hirotake, Senju Satoru, Fukuma Daiki, Yuba Eiji, Kono Kenji, Yoshida Koji, Nakamura Yusuke, Nakayama Hideki and Nishimura Yasuharu
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] Identification of promiscuous oncofetal antigen (IMP-3)-derived long peptides, bearing both Th cells and CTL epitopes (2015)
  • Author(s): Hirayama Masatoshi, Tomita Yusuke, Yuno Akira, Mohammad Abu Sayem, Tsukamoto Hirotake, Irie Atsushi, Senju Satoru, Yoshitake Yoshihiro, Fukuma Daiki, Shinohara Masanori , Yuba Eiji, Kono Kenji, Yoshida Koji, Nakamura Yusuke, Nakayama Hideki and Nishimura Yasuharu
  • Organizer: American Association for Cancer research (AACR) Annual Meeting 2015
  • Place of Presentation: フィラデルフィア
  • Year and Date: 2015-04-18
  • Int'l Joint Research
• [Presentation] 新規癌精巣抗原KIF-20A由来のCTLとTh1細胞を活性化する、がんペプチドワクチンの同定 (2015)
  • Author(s): 湯野晃、冨田雄介、平山真敏、福間大喜、吉武義泰、尾木秀直、中山秀樹、平木昭光、中村 祐輔、西村泰治、篠原 正徳
  • Organizer: 第33回日本口腔腫瘍学会総会
  • Place of Presentation: 奈良
  • Year and Date: 2015-01-29 – 2015-01-30
• [Presentation] 腫瘍関連抗原特異的なCTLとTh1細胞を活性化する、がんペプチドワクチン療法の開発 (2014)
  • Author(s): 湯野晃、冨田雄介、平山真敏、福間大喜、吉武義泰、中山秀樹、平木昭光、中村祐輔、西村泰治、篠原正徳
  • Organizer: 第59回日本口腔外科学会総会
  • Place of Presentation: 千葉
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] Tumor-associated antigen-specific CD4+ T-cell immunity augmented by CTL-epitopes vaccination in patients with malignant tumor (2014)
  • Author(s): Yuno Akira, Tomita Yusuke, Hirayama Masatoshi, Fukuma Daiki, Yoshitake Yoshihiro, Ogi Hidenao, Nakayama Hideki, Hiraki Akimitsu, Tsunoda Takuya, Daigo Yataro, Nakamura Yusuke, Nishimura Yasuharu, Shinohara Masanori
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27