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Transcriptional regulation of XI/XXVII collagen gene in chondrocyte

Research Project

Project/Area Number 26893208
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionOita University

Principal Investigator

Hida Mariko  大分大学, 医学部, 客員研究員 (10737224)

Project Period (FY) 2014-08-29 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords軟骨 / コラーゲン / 転写
Outline of Final Research Achievements

We investigated the proximal promoter of mouse Col11a1 gene in chondrocytes. Cell transfection experiments with reporter constructs in the absence of NF-Y binding sequence exhibited the suppression of the promoter activity. Furthermore, NF-Y protein directly bound to this region in vivo, and dominant-negative NF-Y A mutant inhibited the promoter activity. Next, we further characterized the proximal promoter activity of mouse Col11a1 gene. Luciferase assays exhibited that GC rich sequence is a critical elements of Col11a1 promoter activity, and the most effective candidate was transcription factor Sp1. Overexpression of Sp1 was significantly increased the activity and knockdown of Sp1 expression was suppressed the proximal promoter activity and the expression of endogenous transcript of mouse Col11a1 gene. Taken together, these results indicate that the transcription factor NF-Y and Sp1 upregulates the proximal promoter activity of mouse Col11a1 gene in chondrocytes.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • Research Products

    (2 results)

All 2016 2015

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (1 results)

  • [Journal Article] 1)Sp1 upregulated the proximal promoter activity of the mouse collagen a1(XI) gene (Col11a1) in chondrocytes.2016

    • Author(s)
      Watanabe K, Hida M, Sasaki T, Yana H, Kawano K, Yoshioka H, Matsuo N.
    • Journal Title

      In Vitro Cell Dev Biol Anim

      Volume: 52 Issue: 2 Pages: 235-42

    • DOI

      10.1007/s11626-015-9959-y

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] XI型コラーゲンα1鎖遺伝子(Col11a1)の転写調節機構の解明2015

    • Author(s)
      渡邊啓次郎、樋田真理子、佐々木隆子、河野憲司、吉岡秀克、松尾哲孝
    • Organizer
      第39回蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      豊泉荘(大分県、別府市)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report

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Published: 2014-09-09   Modified: 2017-05-10  

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