Development of a technique for reconstructing bone defects after resection of malignant musculoskeletal tumors using tissue engineering.

• Project/Area Number: 26893238
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Nara Medical University
• Principal Investigator: Uchihara Yoshinobu 奈良県立医科大学, 医学部, 助教 (60736924)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 再生医療

Outline of Final Research Achievements

The aim of this study is to create a new technique for reconstructing bone defects after resection of malignant musculoskeletal tumors. Extracorporeal autogenous devitalized bone grafting is a treatment option for bone reconstruction. However, nonunion often occurs because the osteogenic capacity is lost by irradiation or liquid nitrogen. We established a devitalized bone graft model in the rat femur to assess whether osteogenic matrix cell sheets improve osteogenesis of the devitalized bone. By combining the debitalized bone graft with an osteogenic matrix cell sheet, bone union was achieved shortly after the operation. In contrast the control group exhibited no bone formation.
Our study indicates that osteogenic matrix cell sheet transplantation might be a powerful method to facilitate osteogenesis in devitalized bones, which may become a treatment option for reconstruction of bone defects after resection of malignant musculoskeletal tumors.

Research Products

• [Journal Article] Osteogenic Matrix Cell Sheets Facilitate Osteogenesis in Irradiated Rat Bone (2015)
  • Author(s): Yoshinobu Uchihara, Manabu Akahane, Takamasa Shimizu, Tomoyuki Ueha, Yusuke Morita, Shintaro Nakasaki, Tomohiko Kura, Yasuaki Tohma, Akira Kido, Kenji Kawate, Yasuhito Tanaka
  • Journal Title: BioMed Research International Volume: 2015 Pages: 1-7
  • DOI: 10.1155/2015/629168
  • Peer Reviewed / Open Access / Acknowledgement Compliant