| Project/Area Number |
26893248
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Prosthodontics/ Dental materials science and
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| Research Institution | Iwate Medical University |
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Principal Investigator |
Jun Yokota 岩手医科大学, 歯学部, 助教 (60733730)
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 遺伝子導入 / 骨再生 / PDGF / TGF-β / MSC |
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| Outline of Final Research Achievements |
In this study, to affect growth factors to the mesenchymal stem cells (MSC), we transfected plasmid vector, and evaluate the osteogenic differentiation. And we also investigated the effects of TGF-β and VEGF on the osteogenic differentiation of MSC. As a result, BMP-2 plasmid vector induced matrix mineralization in the MSC in a dose-dependent manner.
TGF-β and VEGF stimulated to the MSC, cell proliferation was downregulated, but osteo differentiation markers (ALP, BSP, OCN) expression was significantly upregulated. Furthermore TGF-β and VEGF induced extracellular signal-regulated kinase (ERK) phosphorylation. These findings indicated that gene transfection and the combination of TGF-β and VEGF were significantly differentiated MSC into osteoblasts, and our findings provide insight into the establishment of novel therapeutic methods for bone formation.
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