| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In the present study, we have examined the mechanism for osteoclast differentiation and inflammatory bone resorption by an osteoclasts differentiation inhibitory peptide, scrapie-responsive gene 1 (SCRG1), derived from mesenchymal stem cells (MSC). Mouse macrophage-like Raw264.7 cells as the osteoclast precursor were investigated activation of the intracellular signal transduction pathways and gene expression analysis after treatment with recombinant mouse SCRG1 (rmSCRG1). As a result, rmSCRG1 was significantly enhanced the phosphorylation of ERK1/2. In addition, mrSCRG1 was promoted the expression of chemokine receptor, CCR7, not only reduced the expression of LPS-induced chemokines, CCL22. Therefore, these results strongly suggested that SCRG1 secreted from MSC in the inflammatory tissues suppress the proinframmation and inflammatory bone resorption through a receptor complex, BST-1/β-integrin on the cell surface and activation of ERK1/2 of macrophages.
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