Analysis of the mechanisms for the inflammatory bone resorption by an osteoclasts differentiation inhibitory peptide derived from mesenchymal stem cells.
| Project/Area Number |
26893249
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
Kikuchi Emiko 岩手医科大学, 歯学部, 研究員 (50733854)
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 間葉系幹細胞 / 破骨細胞 / 炎症性骨吸収 |
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| Outline of Final Research Achievements |
In the present study, we have examined the mechanism for osteoclast differentiation and inflammatory bone resorption by an osteoclasts differentiation inhibitory peptide, scrapie-responsive gene 1 (SCRG1), derived from mesenchymal stem cells (MSC). Mouse macrophage-like Raw264.7 cells as the osteoclast precursor were investigated activation of the intracellular signal transduction pathways and gene expression analysis after treatment with recombinant mouse SCRG1 (rmSCRG1). As a result, rmSCRG1 was significantly enhanced the phosphorylation of ERK1/2. In addition, mrSCRG1 was promoted the expression of chemokine receptor, CCR7, not only reduced the expression of LPS-induced chemokines, CCL22. Therefore, these results strongly suggested that SCRG1 secreted from MSC in the inflammatory tissues suppress the proinframmation and inflammatory bone resorption through a receptor complex, BST-1/β-integrin on the cell surface and activation of ERK1/2 of macrophages.
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Report
(3 results)
Research Products
(7 results)
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[Journal Article] Novel SCRG1/BST1 axis regulates self-renewal, migration, and osteogenic differentiation potential in mesenchymal stem cells.2014
Author(s)
Aomatsu, E., Takahashi, N., Sawada, S., Okubo, N., Hasegawa, T., Taira, M., Miura, H., Ishisaki, A., and Chosa, N.
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Journal Title
Sci. Rep.
Volume: 4
Issue: 1
Pages: 3652-3652
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Cell-cell adhesion through N-cadherin enhances VCAM-1 expression via PDGFRβ in a ligand-independent manner in mesenchymal stem cells.2014
Author(s)
Aomatsu, E., Chosa, N., Nishihira, S., Sugiyama, Y., Miura, H., and Ishisaki, A.
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Journal Title
International Journal of Molecular Medicine
Volume: 33
Issue: 3
Pages: 565-572
DOI
Related Report
Peer Reviewed
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