Project/Area Number |
26893253
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
Virology
|
Research Institution | Saitama Medical University |
Principal Investigator |
ohta naoyuki 埼玉医科大学, 医学部, 助教 (00714255)
|
Project Period (FY) |
2014-08-29 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | gene therapy / naive PS cells / HDAdV / ナイーブPS細胞 / アデノウイルスベクター / HDAdベクター / ヒトナイーブPS細胞 |
Outline of Final Research Achievements |
The purpose of this study is to develop a method for highly efficient gene targeting. To achieve the goal, in this study, human naive pluripotent stem cells were infected with a helper dependent adenovirus vector (HDAdV). A method to induce human naive pluripotent stem (PS) cells from primed induced pluripotent stem (iPS) cells has been previously reported (Chan et al., Cell Stem Cell, 2013). Using this method, we performed the induction of human naive pluripotent stem cells. Gene targeting was performed to these cells using HDAdV. However, efficiency of gene targeting in naive PS cells has not been higher than that in primed iPS cells. In order to improve the method, we tried to develop a method for feeder-free culture of naive PS cells. In the future, we perform gene targeting in feeder-free naive PS cells using HDAdV.
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