| Project/Area Number |
26893273
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Showa University |
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Principal Investigator |
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| Research Collaborator |
上條 竜太郎 昭和大学, 歯学部口腔生化学講座, 教授 (70233939)
須澤 徹夫 昭和大学, 歯学部口腔生化学講座, 講師 (60271285)
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 神経堤由来細胞 / 硬組織再生 / 細胞移植 / 再生医療 / 骨芽細胞 |
|---|
| Outline of Final Research Achievements |
We isolated neural crest-derived cells from nasal concha of adult P0-Cre/CAG-CAT-EGFP mice and cultured in adhesive cell-culture plates in the presence of BMP-2, an inducer of osteoblastic differentiation. We confirmed osteoblastic differentiation using alkaline phosphatase activity staining, alizarin red staining and real-time PCR analysis. Also, lipid droplets were observed in adipogenic stimulated cultures using oil red O staining. From the above mentioned results, it is suggested that neural crest-derived cells isolated from nasal concha of adult P0-Cre/CAG-CAT-EGFP mice were multipotent and capable of differentiating osteoblasts and adipocytes.
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