Involvement of apoptosis of cementocytes and cementoblasts in inducement of odontoclastogenesis
| Project/Area Number |
26893289
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
SHIMIZU Mami 日本大学, 松戸歯学部, 助手(専任扱) (50732856)
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 歯根吸収 / アポトーシス / セメント質 / セメント芽細胞 / 矯正力 / 破歯細胞 |
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| Outline of Final Research Achievements |
Twelve male 6-week-old Wistar rats were subjected to orthodontic force of 10 or 50 g to induce a mesially tipping movement of the upper first molars for 5 days. The expression levels of caspase-3, 8 protein were determined in cementum area by Immunohistochemical analysis. Furthermore, the effect of compression force on the expression of caspase-3, 8 were investigated using human cementoblasts in vitro. As a result, in vivo study revealed an increased caspase-8 -positive cells in 50g orthodontic force groups on day 5. The immunoreactivity for caspase-8 in 50g group was observed in resorbed root surface subjected to the orthodontic force. In the in vitro study, the compression force increased the expression of caspase-3, 8 from the human cementoblasts on 3 hours, and reached the peak on 6 hours. The results of this study suggest that apoptosis response to excessive orthodontic force may aggravate the process of root resorption in human cementoblasts.
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Report
(3 results)
Research Products
(12 results)
