The innovative evaluation of the alveolar ridge at the site of dental implant placement.

• Project/Area Number: 26893305
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Osaka Medical College
• Principal Investigator: KANOH Miwa 大阪医科大学, 医学部, 非常勤医師 (60738269)
• Research Collaborator: UENO Takaaki 大阪医科大学, 医学部, 教授 (60252996)
• Project Period (FY): 2014-08-29 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 歯科インプラント / 歯槽骨 / 組織 / 非脱灰凍結切片作製法 / 歯科インプント / 骨質 / 非脱灰凍結切片 / 組織学的観察 / 迅速評価

Outline of Final Research Achievements

The evaluation of bone quality at the site of the alveolar bone for a dental implant is very important. This study presents an easy technique for direct evaluation of alveolar bone quality using nondecalcified cryofilm frozen sections on human alveolar ridge bone core samples.The forty core samples harvested from alveolar bone were immediately frozen in cooled hexanen and slowly cut using a disposable tungsten carbide blade the sliced sections were collected with adhesive cryofilms. Staining was performed using toluidine blue and von Kossa for microscopic observations.All core samples clearly showed bone structure components of cortical bone, trabecular bone, bone marrow, blood vessels and bone-related cells. The rate of hard tissue in the whole area of the core samples were able to be measured by computer image analyzed system.These results suggest the efficacy of a nondecalcified cryofilm frozen section technique for histological observation of surgical implant sites.

Research Products

• [Journal Article] Rapid and easy histological evaluation of albeolar human bone quality at dental implant sites using a non-decalcified frozen cryofilm section technique (2015)
  • Author(s): Yuichi Ito, Hiroshi Fujita, Miwa Knou, Yasuko Nakagawa, Yoichiro Nakajima, Akihiro Sunano, Yasuhiro Kimura, Takaaki Ueno
  • Journal Title: Implant Dentistry Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Quantification of growth factors in platelet-rich fibrin (2015)
  • Author(s): Nozomu Fukui, Takaaki Ueno, Yuichi Ito, yasuko Takahashi, Yoshihiro Kimura, Yoichiro Nakajima, Shin Kasuya, Miwa Kanou, Kimiko Takubo, Kayoko Yamamoto, Yoshihiro Suwa, Akihiro Sunano, Norihiro Hashiguchi
  • Journal Title: Journal of Hard Tissue Biology Volume: 印刷中
  • Peer Reviewed
• [Presentation] PRP由来のTGF-β1、VEGF濃度に関する研究 (2015)
  • Author(s): 木村吉宏、植野高章、福居 希、加須屋 真、高橋泰子、寺井陽彦、加納みわ、砂野彰宏、田中芳人
  • Organizer: 第６９回日本口腔科学会学術集会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2015-05-13 – 2015-05-15