Analysis of GABAergic neuron using Dravet symdrom patients iPS Cell

• Project/Area Number: 26893318
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Pediatrics
• Research Institution: Fukuoka University
• Principal Investigator: KAWANO HIROYUKI 福岡大学, てんかん分子病態研究所, ポスト・ドクター (10736218)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: てんかん / Dravet症候群 / iPS細胞 / シナプス

Outline of Final Research Achievements

The goal of this research is to investigate GABAergic transmission and try to detect its disfunction using autaptic culture of iPS cells made from the patients of the severe epilepsy, Dravet syndrome. But I can not get adequate response from the autaptic synapse on iPS cells.
Then, in this study I used model mice of Dravet syndrome and discover that the GABAregic synaptic transmission of DS model mice was lowered but glutamatergic synaptic transmission was not change.