Understanding for the induction mechanisms of intestinal IL22-producing cells

• Project/Area Number: 26893326
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Tanoue Takeshi 国立研究開発法人理化学研究所, 統合生命医科学研究センター, 基礎科学特別研究員 (60732972)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: IL22 / 腸内細菌 / 腸内常在菌 / IL-22

Outline of Final Research Achievements

In this study, I focused on interleukin (IL)22-producing cells, which are considered to be a key player in protection against gastrointestinal infection. First, I’ve analyzed the numbers of IL22-prodcing cells in various tissues of healthy mice. As a result, IL22-producing cells were abundant in gut, especially in the small intestine. In contrast to microbiota-harboring SPF (specific pathogen free) mice, germ-free mice showed substantially lower numbers of the cells, suggesting gut microbiota induce the accumulation of IL22-producing cells. Furthermore, I found that type3 innate lymphoid cells are main cellular source of IL22 and gram-positive ingenious members induce the IL22+ ILC3.

Research Products

• [Journal Article] Th17 Cell induction by adhesion of microbes to intestinal epithelial cells (2015)
  • Author(s): Atarashi, K., Tanoue, T., Ando. M., Kamada, N., Nagano, Y., Narushima, S., Suda, W., Imaoka, A., Setoyama, H., Nagamori, T., Ishikawa, E., Shima, T., Hara, T., Kado, S., Jinnohara, T., Ohno, H., Kondo, T. et al.
  • Journal Title: Cell Volume: 163 Issue: 2 Pages: 367-380
  • DOI: 10.1016/j.cell.2015.08.058
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Microbiota-dependent induction of IL-22-producing ILC3 in the gut (2014)
  • Author(s): Takeshi TANOUE, Koji ATARASHI, Yuji NAGANO, Seiko NARUSHIMA, Yoshinori UMESAKI, Kenya HONDA
  • Organizer: The 43rd Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2014-12-12