In vivo live imaging-based investigation of mural cell-dynamics involved in vascular stability

• Project/Area Number: 26893336
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: Ando Koji 国立研究開発法人国立循環器病研究センター, 研究所, 流動研究員 (10736010)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 壁細胞 / in vivo イメージング / ゼブラフィッシュ / in vivoイメージング

Outline of Final Research Achievements

We clarified the mechanism how mural cells (MCs) develop and cover endothelial cells (ECs) by generating the transgenic zebrafish lines that allow live imaging of MCs and by lineage tracing in vivo. To cover cranial vessels, MCs derived from either neural crest or mesoderm emerged around the ECs, proliferated and migrated along EC tubes. During their migration, the MCs moved forward by extending their processes along the inter-EC junctions, suggesting the role of inter-EC junctions as a scaffold for MC migration. In the trunk vasculature, MCs derived from mesoderm covered the ventral side of the dorsal aorta (DA), but not the posterior cardinal vein. Furthermore, the MCs migrating from the DA or emerging around intersegmental vessels (ISV) preferentially covered arterial ISVs rather than venous ISVs, indicating that MCs mostly cover arteries during vascular development.

Research Products

• [Int'l Joint Research] Dept. of Biol. and Biochem., Bath univ.(United kingdom)
• [Int'l Joint Research] Dept. of IGP, Uppsala univerdity.(Sweden)
• [Journal Article] Clarification of mural cell coverage of vascular endothelial cells by live imaging of zebrafish. (2016)
  • Author(s): Ando K, Fukuhara S, Izumi N, Nakajima H, Fukui H, Kelsh RN, Mochizuki N.
  • Journal Title: Development Volume: 143 Pages: 1328-1339
  • DOI: 10.1242/dev.132654
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Recent advances in understanding of cardiovascular development by imaging of zebrafish. (2015)
  • Author(s): Fukuhara S, Fukui H, Wakayama Y, Ando K, Nakajima H, Mochizuki N.
  • Journal Title: Dev Growth Differ. Volume: 未定 Issue: 4 Pages: 109-122
  • DOI: 10.1111/dgd.12210
  • Peer Reviewed / Open Access
• [Journal Article] RhoA and ROCK mediate histamine-induced vascular leakage and anaphylactic shock. (2015)
  • Author(s): Mikelis CM, Simaan M, Ando K, Fukuhara S, Sakurai A, Amornphimoltham P, Masedunskas A, Weigert R, Chavakis T, Adams RH, Offermanns S, Mochizuki N, Zheng Y, Gutkind JS.
  • Journal Title: Nat. Commun. Volume: 6 Issue: 1 Pages: 6725-6725
  • DOI: 10.1038/ncomms7725
  • Peer Reviewed
• [Journal Article] β-Catenin-dependent transcription is central to Bmp-mediated formation of venous vessels. (2015)
  • Author(s): Kashiwada T, Fukuhara S (Co-corresponding author), Terai K, Tanaka T, Wakayama Y, Ando K, Nakajima H, Fukui H, Yuge S, Saito Y, Gemma A, Mochizuki N.
  • Journal Title: Development Volume: 142 Pages: 497-509
  • DOI: 10.1242/dev.115576
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cdc42 mediates Bmp-induced sprouting angiogenesis through Fmnl3-driven assembly of endothelial filopodia in zebrafish (2015)
  • Author(s): Wakayama Y, Fukuhara S, Ando K, Matsuda M and Mochizuki N.
  • Journal Title: Developmental Cell Volume: 32 Pages: 109-122
  • Peer Reviewed / Open Access
• [Journal Article] Visualizing the cell-cycle progression of endothelial cells in zebrafish. (2014)
  • Author(s): Fukuhara S (Co-corresponding author), Zhang J, Yuge S, Ando K, Wakayama Y, Sakaue-Sawano A, Miyawaki A, Mochizuki N.
  • Journal Title: Dev. Biol. Volume: 393 Issue: 1 Pages: 10-23
  • DOI: 10.1016/j.ydbio.2014.06.015
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Ｌive-imaging of mural cells in zebrafish (2015)
  • Author(s): Koji Ando, Shigetomo Fukuhara, Naoki Mochizuki
  • Organizer: Gordon research seminor and conference
  • Place of Presentation: Salve Regina University Newport, RI, USA
  • Year and Date: 2015-07-31
  • Int'l Joint Research
• [Presentation] Live-imaging of mural cells in zebrafish (2015)
  • Author(s): Ando K, Fukuhara S and Mochizuki N.
  • Organizer: 第9回 Aso international meeting
  • Place of Presentation: ホテルグリーンピア南阿蘇
  • Year and Date: 2015-05-14 – 2015-05-16
• [Presentation] In vivo live-imaging of mural cells in zebrafish (2015)
  • Author(s): Ando K, Fukuhara S and Mochizuki N.
  • Organizer: 春期特別日本血管生物医学会シンポジウム
  • Place of Presentation: 大阪大学微生物学研究所
  • Year and Date: 2015-05-13
• [Funded Workshop] Gordon research seminar and conference (2015)
  • Place of Presentation: Salve Regina University Newport, RI, USA
  • Year and Date: 2015-07-31