| Project/Area Number |
56480242
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
NISHIMOTO Akira OKAYAMA UNIVERSITY MEDICAL SCHOOL PROFESSOR, 医学部, 教授 (50032850)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIWARA Takashi OKAYAMA UNIVERSITY HOSPITAL SENIOR RESIDENT, 医学部・附属病院, 医員
FURUTA Tomohisa OKAYAMA UNIVERSITY HOSPITAL SENIOR RESIDENT, 医学部・附属病院, 医員
MATSUMOTO Kengo OKAYAMA UNIVERSITY HOSPITAL SENIOR RESIDENT, 医学部・附属病院, 医員
NORIKANE Hiroshi OKAYAMA UNIVERSITY HOSPITAL SENIOR RESIDENT, 医学部・附属病院, 医員
HARADA Yasuhiro OKAYAMA UNIVERSITY HOSPITAL ASSITANT, 医学部, 助手 (90135947)
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| Project Period (FY) |
1981 – 1983
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| Project Status |
Completed (Fiscal Year 1983)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1983: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1982: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1981: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | DIFFERNTIAL HYPOTHERMIA / BRAIN TUMOR CELLS / ACNU |
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| Research Abstract |
Effect of Differential Hypothemia on Cultured Brain Tumor Cells
1. The effects of combined use of heat with drug were studied by colony forming assay, using a cultured rat glioma cell line (C6). The cells were treated with a nitrosourea derivative ACNU in different thermal conditions.
After treament of 43ﾟC for 1 or 2 hours, the surviving fraction reduced to 76% or 28% of non-treatment cells. While with 5mug/ml ACNU,the surviving fraction reduced to 55% or 19% and with 20mug/ml ACNU, 3% or 0.5%, respectively. These results proved that hyperthermialead to marked increase of cytocidal effect of ACNU and suggested the synergestic activity of ACNU and differential hypothermia to achieve greater tumoricidal effect.
2. An experimental brain tumor model was established with VX2 tumor cells stereotactically transplanted in the rabbit brain. On the other hand, to investigate the thermal distribution in the normal brain under 915MHz microwave irradiation, the thermal distribution under 915MHz and 2.450MHz microwave irradiation were compared. More uniform and relatively deeper heeting was achieved with 915MHz microwave irradiation than with 2.450MHz. However, it seemed quite difficult for the generator presently used for the 915MHz irradiation, whose maximal generating power is 45W,to keep the brain over 37ﾟC under the systemic hypothermia of 30ﾟC.Therefore, reinforcement of the generator seemed to be necessary. Furthrmore, further heating effect could achieved to fill aluminium oxide between the microwave antenna and the object than air gap. No macro-or microscopically pathologic change was seen after 5-hour continuous heating of the brain under systemic hypothermia of 30ﾟC.
3. To evaluate the tumoricidal effect of differential hypothermia, a Rous Sarcoma virus-induced brain tumor model was established in adult monkeys
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