• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on Biological and Physical States of Membrane Lipids in the Adaptational Changes of Staphylococcus Aurous

Research Project

Project/Area Number 57480172
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionOkayama University

Principal Investigator

KANEMASA Yasuhiro  Okayama University Medical School, Department of Microbiology, Professor, 医学部, 教授 (80033059)

Co-Investigator(Kenkyū-buntansha) KARIYAMA Reiko  Okayama University Medical School, Department of Microbiology, Research Associat, 医学部, 助手 (40112148)
HIRAI Yoshikazu  Okayama University Medical School, Department of Microbiology, Research Associat, 医学部, 助手 (00127581)
TOMOCHIKA Ken-ichi  Okayama University Medical School, Department of Microbiology, Lecturer, 医学部, 講師 (00093691)
Project Period (FY) 1982 – 1983
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1983: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1982: ¥4,300,000 (Direct Cost: ¥4,300,000)
KeywordsStaphylococcus / Autoplastic / L-form / Membrane / Lipid / Differential Scanning Calorimetry / Phase Transition Temperature / Surface Charge
Research Abstract

The bacteria alter their membranes adaptively according to changes of environment and cell structure. We have reported the results that staphylococcal autoplasts synthesized predominantly cardiolipin in their membranes and staphylococcal L-forms synthesized cholesterol newly in addition to an increase of cardiolipin. We have done further study toward clarification of these appearances from the viewpoints of biophysics as well as biochemistry.
In order to elucidate why cardiolipin markedly increases in S. aureus cells which lack cell walls, the phase transition characteristics of cardiolipin and phosphatidylglycerol suspensions were investigated by differential scanning calorimetry. The phase transition temperatures for dimyristoylphosphatidylglycerol, tetramyristoylcardiolipin, dipalmitoylphosphatidylglycerol and tetrapalmitoylcardiolipin were 25.0, 47.0, 40.5 and 62.2^゚C, respectively. The phase transition temperature for a mixture of two analogous phospholipids was higher than that fo … More r phosphatidylglycerol alone, but lower than that for cardiolipin alone. It increased along with cardiolipin content. The phase transition temperature for cardiolipin was increased in the presence of divalent cations, particularly Ca^<2+>. The results indicate that the head group of cardiolipin by itself can increase the phase transition temperature.
Moreover, we checked anti-osmotic stability of liposomes prepared with lipids extracted from normal S. aureus, its L-form and Mycoplasma orale, using the release of carboxyfluorescein trapped in liposomes as an indicator. The trapped carboxyfluorescein was released when the liposomes burst upon the inflow of excess water. Liposomes prepared with the lipids of L-form or Mycoplasma were more resistant to osmotic pressure than those prepared from normal S. aureus. It was found that cardiolipin enhanced the membrane-stability in S. aureus and cholesterol in Mycoplasma.
The surface charge of phosphatidylserine liposome and six kinds of bacteria were determined by colloid titration. The amino group was blocked completely by formalin treatment and the negative charge of phosphate and carboxyl group was titrated directly. The amino group can be estimated from the titration difference in the presence and absence of formalin. By this colloid titration method the surface charge can be evaluated stoichrometrically at every pH value, and by formalin treatment the effect of the amino group can be detected quantitatively. Less

Report

(1 results)
  • 1990 Final Research Report Summary
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Yasuko Noda,Takeshi Katayama,Yoshikazu Hirai,Hideo Hayashi and Yasuhiro Kanemasa: "Detection of functional group on the liposome surface by colloid titration" Physiological Chemistry and Physics. 14(4). 375-379 (1982)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Reiko Kariyama: "Increse of cardiolipin content in Staphylococcus aureus by the use of antibiotics affecting the cell wall" The Journal of Antibiotics. 35(12). 1700-1704 (1982)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 狩山 玲子: "黄色ブドウ球菌の壁欠落にともなう膜リン脂質組成変動のモデル解析" 岡山医学会雑誌. 95(3,4). 295-303 (1983)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 内藤 伸明,友近 健一,口分田 晃,塩出 純二,金政 泰弘: "大腸菌のペニシリン誘導フィラメント細胞におけるアミノ酸能動輸送能変化" 岡山医学会雑誌. 95(5,6). 575-581 (1983)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuko Noda,Takeshi Katayama and Yasuhiro Kanemasa: "Determination of surface charge of Micrococcus luteus by colloid titration" Physiological Chemistry and Physics and Medical NMR. 16(1). 29-34 (1984)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 平井 義一,中村 知明,金谷 誠久,志田 健太郎,福原 明宏,金政 泰弘: "ブドウ球菌L型菌のフリ-ズフラクチャ-法による解析" 岡山医学会雑誌. 96(5,6). 631-639 (1984)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Eiko Nagamachi,Reiko Kariyama and Yasuhiro Kanemasa: "The effect of head group structure on phase transition of phospholipid membranes as determined by differential scanning calorimetry" Physiological Chemistry and Physics and Medical NMR. 17(3). 255-260 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuko Noda and Yasuhiro Kanemasa: "Determination of surface charge of some bacteria by colloid titration" Physiological Chemistry and Physics and Medical NMR. 16(4). 263-274 (1984)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 金政 泰弘: "「微生物と環境」微生物学読本(福見 秀雄編)" 日本評論社, 216 (1982)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuko Noda, Takeshi Katayama, Yoshikazu Hirai, Hideo Hayashi and Yasuhiro Kanemasa: "Detection of functional group on the liposome surface by colloid titration" Physiological Chemistry and Physics. 14 (4). 375-379 (1982)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Reiko Kariyama: "Increase of Cardiolipin Content in Staphylococcusaureus by the Use of Antibiotics Affecting the Cell Wall" The Journal of Antibiotics. 35 (12). 1700-1704 (1982)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Reiko Kariyama: "Model Studies on the Alteration of the Phospholipid Composition of Staphylococcus Aurous in Response to the Lack of the Cell Wall" Okayama Igakkai Zasshi. 95 (3, 4). 295-303 (1983)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nobuaki Naito, Ken-ichi Tomochika, Akira Kumode, Junji Shiode and Yasuhiro Kanemasa: "A Defect in Amino Acid Transport of Filamentous Escherichia Coli Induced by Penicillin" Okayama Igakkai Zasshi. 95 (5, 6). 575-581 (1983)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuko Noda, Takeshi Katayama and Yasuhiro Kanemasa: "Determination of Surface Charge of Micrococcus Luteus by Colloid Titration" Physiological Chemistry and Physics and Medical NMR. 16 (1). 29-34 (1984)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoshikazu Hirai, Tomoaki Nakamura, Tomohisa Kanatani, Kentaro Shida Akihiro Fukuhara and Yasuhiro Kanemasa: "Ultrastructure of Staphylococcal L-form cell Membrane by Freeze-fracture replica" Okayama Igakkai Zasshi. 96 (5 , 6). 631-639 (1984)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Eiko Nagamachi, Reiko Kariyama and Yasuhiro Kanemasa: "The Effect of Head Group Structure on Phase Transition of Phospholipid Membranes as Determined by Differential Scanning Calorimetry" Physiological Chemistry and Physics and Medical NMR. 17 (3). 255-260 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuko Noda and Yasuhiro Kanemasa: "Determination of Surface Charge of Some Bacteria by Colloid Titration" Physiological Chemistry and Physics and Medical NMR. 16 (4). 263-274 (1984)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi