Molecular analysis of T cell antigen receptors and functional molecules.
| Project/Area Number |
58440033
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Chiba University |
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Principal Investigator |
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| Project Period (FY) |
1983 – 1985
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| Project Status |
Completed (Fiscal Year 1985)
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| Budget Amount *help |
¥27,200,000 (Direct Cost: ¥27,200,000)
Fiscal Year 1985: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1984: ¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 1983: ¥12,200,000 (Direct Cost: ¥12,200,000)
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| Keywords | Anti-Id suppressor Tcell / Internal Image / アルファ鎖遺伝子 / 抑制T細胞抗原レセプター / 抑制因子 |
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| Research Abstract |
We attempted to analyse immunological roles of anti-idiotypic (Id) receptor on suppressor T cells (Ts) and their molecular genetic properties by using inducible KLH specific anti-Id Ts hybridoma make by fusion of BW5147 and C57BL/6 KLH-Ts. The following observations and conclusions were oftained.
1. Anti-Id Ts receptor works as an regulatory internal image (stereocopy) of the external antigen predominantly seen by suppressor families.
2. The evidence that the internal image is composed of the Ts receptor V region is obtained by the following four experiments:
a) Anti-Id Ts hybridoma expresses the stereocopy of the external antigenic epitope (KLH-like determinant) on the cell surface.
b) The particular anti-KLH antibody reacts with the stereocopy and also possesses the activity to suppress the responses in the KLH specific and H- <2^b> restricted manner.
c) The phenotypic expression of the KLH-like determinant on Ts is changed under chimera conditions.
d) The rat antibody against anti-Id Ts hybridoma reacts with KLH and KLH-specific and mediated H- <2^b> restricted suppressor activity.
3. The paradox of I-J has been solved by using x-irradiation bone marrow chimera. The I-J determinant is found to be the idiotypic structure on Ts.
4. The suppressor factor derived from anti-Id Ts hybridoma is analysed by FPLC system and found to be the 30 KD molecule with pI 6.1-6.2.
5. We have also been successful in cloning and sequencing of Ts alpha chain gene (cDNA) which is found to be active in Ts hybridomas. However, Ts receptor molecules seem to have different configulation from those on Th and Tc, because Ts beta chain genes in Ts hybridomas are all deleted. An undefined forth chain is proposed to exist in association with Ts alpha chain.
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Report
(1 results)
Research Products
(10 results)
