Electrophysiological and electronmicroscopic studies on distribution of the nerve fibers in the guinea pig cochlea
Project/Area Number |
58480344
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Research Category |
Grant-in-Aid for General Scientific Research (B)
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Allocation Type | Single-year Grants |
Research Field |
Otorhinolaryngology
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Research Institution | Hokkaido University |
Principal Investigator |
TERAYAMA Yoshihiko Hokkaido University, School of Medicine. Professor, 医学部, 教授 (90000976)
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Co-Investigator(Kenkyū-buntansha) |
KATO Masamichi Hokkaido University, School of Medicine. Professor, 医学部, 教授 (70000940)
MATSUSHIMA Junichi Hokkaido University, Medical Hospital. Assistant Instructor, 医学部附属病院, 助手 (60173829)
YAMAKAWA Munetaka Hokkaido University, Medical Hospital. Lecturer, 医学部附属病院, 講師 (70002334)
SAKAI Noboru Hokkaido University, Medical Hospital. Lecturer, 医学部附属病院, 講師 (10002271)
TANAKA Katsuhiko Hokkaido University, School of Medicine. Associate Professor, 医学部, 助教授 (10004946)
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Project Period (FY) |
1983 – 1985
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Project Status |
Completed (Fiscal Year 1985)
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Budget Amount *help |
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1985: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1984: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1983: ¥3,300,000 (Direct Cost: ¥3,300,000)
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Keywords | Cochlear nerve / Spiral genglion cells / HPR solution / Sylocain / Aspirin / Superior cervical sympathetic ganglion / 内耳交感神経 |
Research Abstract |
The cochlea is innervated by the afferent cochlear nerve, efferent olivo-cochlear bundle and sympathetic nerve. The purposes of this study are to reveal the distribution and course of the nerve fibers belonging to these nerves in the cochlea by means of elctro-physiological and electron microscopic histochemical methods. As the electrophysiological results, the spiral gauglion cells showed the mean spontaneous discharge rate of 48.9 spikes/sec (SD 30.5). The interspike histograms, spontaneneous discharge rate, variant coeficient of the ganglion were as same as those obtained from the single cochlear nerve fibers. Furthermore, influence of iv .injection of xylocain and aspirin on the spontaneous discharge and spikes evoked by elctric stimulations was observed. The observation indicates that both xylocain and aspirin directly act not only on the hair cells but also on the cochlear nerve fibers. As histochemical and electron microscopic studies, HRP was successfully injected into the cytopl
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asm of the spiral ganglion. But it was failed to histologically follow the nerve fibers stained by HRP. In order to reveal the origin of the sympathetic nerve fibers distributing the cochlea and related arteries, HRP solution was injected into the scala tympani of the guinea pig. Histochemical light microscopical examination of the cervical sympathetic ganglions showed scattered ganglion cells in the ipsilateral superior cervical sympathetic ganglion. On the contrary, the perivascular unmyelinated nerve fibers along the anterior inferior cerebellar artery and labyrinthine artery, and unmyelinated nerve fibers within the osseous spiral lamina electron-microscopically showed dense HRP-granules within their axons in the ipsilateral cochlea. These findings made it evident that ipsilateral superior ganglion is the origin of the sympathetic nerve fibers in the cochlea. Since the spiral ganglion cells are surrounded by double bony shells in the cochlea, injection of HRP following electrophysiological measurement is very difficult. We are still challenging this problem. Less
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Report
(1 results)
Research Products
(10 results)