| Project/Area Number |
58860015
|
|---|
| Research Category |
Grant-in-Aid for Developmental Scientific Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
発酵・醸造
|
|---|
| Research Institution | Yamaguchi University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
1983 – 1985
|
| Project Status |
Completed (Fiscal Year 1985)
|
| Budget Amount *help |
¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1985: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1984: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1983: ¥3,100,000 (Direct Cost: ¥3,100,000)
|
|---|
| Keywords | Pyrroloquinoline quinone / Quinoproteins / 補酵素の発酵生産 / PQQの精製 |
|---|
| Research Abstract |
The possibility of preparing pyrroloquinoline quinone (PQQ), a novel coenzyme, by a fermentative method was proposed and established a simple method for PQQ fermentation. PQQ was formed and accumulated in the culture medium of various microorganisms. Some methylotrophs were most favorable for PQQ production and about 100 ug/ml of PQQ was found in the culture medium after 2-3 days incubation. Methanol was utilized effectively for PQQ formation and 1-3% of methanol in the culture medium was found to be optimal. It was also found that the regulation in PQQ formation was accompanied with magnesium concentration of the medium.
An attempt at the isolation and purification of PQQ was made successfully and spectral evidence was obtained suggesting that the isolated compound was identical with authentic PQQ.
By the way, an improved enzymatic method for the determination of PQQ was developed with cytoplasmic membrane of Escherichia coli, in which D-glucose dehydrogenase (EC 1.1.99.17), a quinoprotein, was completely resolved to apoenzyme by EDTA treatment. Incubation of the EDTA-treated membrane with exogenous PQQ by assaying the restored D-glucose dehydrogenase activity.
|
