| Project/Area Number |
58870029
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | Nihon University (1985)
国立予防衛生研究所 (1983-1984) |
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Principal Investigator |
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| Project Period (FY) |
1983 – 1985
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| Project Status |
Completed (Fiscal Year 1985)
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| Budget Amount *help |
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1985: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1984: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1983: ¥3,500,000 (Direct Cost: ¥3,500,000)
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| Keywords | Non-A / non-B hepatitis / Monoclonal antibody |
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| Research Abstract |
An attempt was made to establish the antigen-antibody system associated with non-A, non-B hepatitis. First we purified antigen reacted with convalescent serum of the non-A, non-B hepatitis patients from the liver of a patient posttransfusion non-A, non-B hepatitis. This antigen designated as AN6520 antigen and established assay system of this antigen and antibody by EIA. Hi-prevalance of antibody in the convalescent srea of non-A, non-B hepatitis was evident. However, further investigation disclosed this antigen-antibody system does not dierctly associated with non-A, non-B hepatitis. The other attempt to clone the B-lymphocyte producing the antibody associated with non-A, non-B hepatitis from convalescent chimpanzee was made by EB virus transformation method. We established monoclonal antibody directed microtubular aggregate which is thought as specific for non-A, non-B infected chimpanzee. Purification of the antigen and establishment of assay system are ongoing.
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