| Budget Amount *help |
¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1984: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1983: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Research Abstract |
Coxsackieviruses are known to be the causes of various diseases. Coxsackievirus <B^4> is considered as the probable cause of juvenile onset type diabetes mellitus and fundamental studies on the development of Coxsackievirus group B vaccine is needed. Diabetogenic potential of Coxsackievirus <B^4> isolates was tested by the appearance of hypoglycemia 2 - 4 days after injection of the virus into SJL/J mice and by the appearance of hyperglycemia late after infection. New virus isolates were divided into two groups according to the induction of hypoglycemia. Virus group which induces hypoglycemia early after infection of SJL/J mice can be subdivided into two, only acinar cells are infected with virus in one group and acinar cells as well as islet cells. One strain which induces hypoglycemia in SJL/J mice and infects islet cells is chosen as the virulent strain. This strain do induce hypoglycemia in other mouse strains ( <C_3> H/He, BALB/c and Jcl/ICR) but infection occurred only in acinar cells. Injection of avirulent strains (strains which do not induce hypoglycemia) prior to infection with the virulent strain protected the mice from the induction of hypoglycemia.
The evidences suppor the ideas written below.
1) The diabetogenic characteristics are attributed to both the virulence of the virus and the genetic properties of the host.
2) Non-pathogenic virus can be used as the vaccine for the pathogenic virus.
3) Before field-trial of a candidate live vaccine, its pathogenicity other than damage of pancreas should be checked.
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