An application of the clinical diagnostic method detecting gene disorders to allergic disease

Research Project

Project/Area Number	58870038
Research Category	Grant-in-Aid for Developmental Scientific Research
Allocation Type	Single-year Grants
Research Field	Immunology
Research Institution	Osaka University
Principal Investigator	NAKAI Sumiko 大阪大学, 医, 助手 (10028508)
Project Period (FY)	1983 – 1985
Project Status	Completed (Fiscal Year 1985)
Budget Amount *help	¥5,100,000 (Direct Cost: ¥5,100,000) Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000) Fiscal Year 1984: ¥1,500,000 (Direct Cost: ¥1,500,000) Fiscal Year 1983: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywords	human Ig E gene / RFLP / アレルギー
Research Abstract	The clinical diagnostic method to detect gene disorders using Southern hybridization technique was tried to apply to allergic disease. The following results were obtained. (1) The polymorphisms in healthy human population were observed in the region from D to C of immmunoglobuline heavy chain genes. Using DNA fragments each of which encodes <C_(epsilon)> , <S_(epsilon)> , D regions as probes, DNAs of peripheral lymphocytes from 96 donors and placentas from 11 individuals were analysed. Bam HI site at 5' of <C_(epsilon 1)> ,Hind II and/or Sac I sites at 5' of <C_(epsilon 2)> , and deletion extended 10 kb which containning Dl encoding sequence were showed polymorphisms. The gene frequencies of the variable sites at <S_(epsilon)> , <C_(epsilon 2)> , and D region were 0.3, 0.3,and 0.5 respectively. (2) DNAs from 27 allergic patients showed no detectable insertion or deletion in the regions of <S_(epsilon)> or <C_(epsilon)> . The gene frequencies of the polymorphic DNA fragments showed no significant difference from healthy human population.