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Ultramicrotracing of Biological Active Substances by High Performance Liquid Chromatography

Research Project

Project/Area Number 58870103
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Physical pharmacy
Research InstitutionKitasato University

Principal Investigator

KINOSHITA Toshio  北里大学, 薬, 教授 (70053816)

Project Period (FY) 1983 – 1985
Project Status Completed (Fiscal Year 1985)
Budget Amount *help
¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1984: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1983: ¥6,000,000 (Direct Cost: ¥6,000,000)
KeywordsHigh performance liquid chromatography / fluorometric analysis / guanidinecontaining peptide / thiol-containing peptide / catecholamine / steroid / bile acid sulfate / 1-ブロモアセチルピレン / 1-アンスロイルニトリル
Research Abstract

Achievements of the project is as follows:
Kinoshita established a method for determination of bradykinine using alkaline ninhydrin reagent and applied it to the studies on enzymatic degradation of gradykinine in human plasma. Nimura established a method for determination of glutathione in human blood using o-phthalaldehyde reagent. Diluted blood could be directly injected into a chromatograph without pre-treatment. Imai developed a method for determination of catecholamines catecholamines and L-dopa using ethylenediamine and applied it to monitor these substances in biological samples. Tsuji synthesized 1-bromoacetylpyrene for the fluorescent labeling of carboxyl group and employed it to estimate sulfateconjugated bile acids in biological fluids. Nambara prepared 9- or 1-anthroylnitrile as a fluorescent labeling reagents for hydroxy group and applied it to the determination of various steroids and bile acids. Ohkura devised a method to determine histidine decarboxilase activity by measuring histamine fluorometrically.
New determination methods established in this research project were extremely effective for the ultramicrotracing of such biological materials.

Report

(1 results)
  • 1985 Final Research Report Summary
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Experientia. 41. (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] Anal.Biochem.146. (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] J.Chromatogr.328. (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] J.Chromatogr.345. (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] J.Chromatogr.311. (1984)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] Experimentia. 41. (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] Anal. Biochem.146. (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] J. Chromatogr.328. (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] J. Chromatogr.345. (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] J. Chromatogr.311. (1984)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1985 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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