| Project/Area Number |
59340049
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物形態・分類学
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| Research Institution | Saitama University |
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Principal Investigator |
URANO Akihisa Faculty of Science, Saitama University, 理学部, 助教授 (00142232)
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| Co-Investigator(Kenkyū-buntansha) |
KIKOVAMA Sakae School of Education, Waseda University, 教育学部, 教授 (20063638)
KATAGIRI Chiaki Faculty of Science, Hokkaido University, 理学部, 教授 (90000827)
OGURO Chitaru Faculty of Science, Toyama University, 理学部, 教授 (40018974)
UEDA Kazuo Faculty of Science, University of Tokyo, 理学部, 教授 (60011441)
IWASAWA Hisaaki Faculty of Science, Niigata University, 理学部, 教授 (60018249)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 1986: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1985: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1984: ¥4,700,000 (Direct Cost: ¥4,700,000)
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| Keywords | Toad / Reproduction / Sexual organ / Reproductive behavior / Endocrine system / Nervous system / 生殖行動 |
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| Research Abstract |
Biological aspects of reproduction in the Japanese toad (Bufo japonicus) were examined by use of multidisciplinary research techniques.
Development and maturation of reproductive organs. Patterns of development and maturation of gonads were examined by morphological techniques.
Endocrine approaches. Adenohypophysial hormones, e.g., prolactin (PRL), follicle stimulating hormone (FSH) and luteinizing hormone (LH), were purified to establish radio immunoassay methods for them. Seasonal variations of various hormones including PRL, FSH and LH measured by RIAs corresponded reproductive activity. In addition, nucleotide sequences of mRNAs for vasotocin and mesotocin were determined by a recombinant DNA technique.
Ethology. Comparisons of ethograms of toad reproductive behavior showed that the toad gradually terminates hibernation and initiates migration to a particular breeding pond. The toad may detect the location of the pond by a memory concerning olfactory cues. Hibernation is not necessitated for maturation of gonads and initiation of reproductive behavior.
Neuroethology. A triggering center for male reproductive behavior was localized in the preoptic nucleus, especially in its anterior part (APON). Mate calling was evoked by an electrical stimulation of the APON. Activities of APON neurons may be motivated by LHRH and TRH, hypothalamic neurohormones.
Developmental chemistry. Jerry coats surrounding eggs are a reservoir for Ca ions required for fertilization.
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