| Project/Area Number |
59370012
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
SUZUKI Fujio Department of Biochemistry, Faculty of Dentistry, Osaka University, 歯学部, 教授 (40028717)
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| Co-Investigator(Kenkyū-buntansha) |
KISHIMOTO Tadamitsu Division of Cellular Immunology, Institute for Molecular and Cellular Biology, O, 細胞工学センター, 教授 (10093402)
ICHIHARA Akira Institute for Enzyme Research Tokushima University, 酵素科学センター, 教授 (40035374)
TAKEDA Yoshiro Department of Biochemistry, School of Dentistry, Tokushima University, 歯学部, 教授 (00028705)
TAKAKU Fumimaro The 3rd Department of Internal Medicine, University of Tokyo, 医学部, 教授 (40048955)
佐藤 威 東北大学, 抗酸菌研究所, 助手 (80125567)
YAMANE Isao Department of Cell Biology, Research Institute for Tuberculosis and Cancer, Toho
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1985: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1984: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Cartilage-Derived Factor (CDF) / Cartilage-derived Anti-Tumor Factor (CATF) / Fibroblast-derived Endothelial Cell Growth Factor (f-ECGF) / Erythropoietin-like factor / Insulin-Stimulating Peptide (ISP) / Platelet-Derived Growth Inhibitor (PDGI) / Tumor Growth Stimulating Activity (TGSA) / Bリンパ球分化因子 |
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| Research Abstract |
F. Suzuki demonstrated that fetal bovine cartilage contains both a somatomedin-like factor on cultured rabbit chondrocytes and an endothelial cell growth inhibitor. He suggested that the cellular response to somatomedin-like factor was highly dependent on the density of chondrocytes.
I. Yamane (T. Satoh) purified fibroblast-derived endothelial cell growth factor (f-ECGF) from serum-free medium of human embryo fibroblasts. f-ECGF is an acidic-glycoprotein with an isoelectric point in the pH range 4.5-6, and Mr approx. 30 kD.
K. Shizume found that human anterior pituitary tissues contain heat- and acid-stable TGF-like mitogen(s) with Mr 15 kD. This factor did not compete with EGF for receptor binding and did not require EGF for colony forming activity.
F. Takaku found that a renal cell carcinoma and a hepatocellular carcinoma produce a marked erythropoiesis in each patient. The erythropoietin-like activities were purified from the conditioned media from both tumor cells.
Y. Takeda found that a two-chain polypeptide, which corresponds to amino acid residues 115-143 and 144-184 (185) of bovine serum albumin, potentiated the effects of insulin on glucose metabolism in isolated rat adipocytes.
A. Ichihara found that rat platelets contain not only a growth factor (Hepatocyte growth factor; HGF), but also at least two types of growth inhibitor (Platele-derived growth inhibitor-alpha; PDGI-alpha, and PDGI-beta) that affect adult rat hepatocytes.
K. Nishikawa purified tumor growth stimulating activity (TGSA) from the rat Rhodamine fibrosarcoma tissue. He suggested that TGSA, a new class of potent growth factor produced by tumor cells, supports their own growth.
T. Kishimoto purified human B-cell differentiation factor (BCDF) to homogeneity from cultured supernatants of TCL-Nal cells. Purified BCDF induced Ig secretion in Epstein-Barr virus-transformed cell lines.
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